NYMU Taipei/Primer List

(Difference between revisions)

Revision as of 15:37, 19 August 2007

Name	Sequence	Length	Tm (<math>\[ ^\user1{O} \user1{C} \] </math>)	Restriction site	Target length	Description
M_INS_A 5'	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG	51 bp	56.85	EcoRI, XbaI	63 bp	Potential dimer formation!
M_INS_A 3'	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT	55 bp	54.74	SpeI, PstI	63 bp	Potential dimer formation!
M_INS_B 5'	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT	52 bp	60.85	EcoRI, XbaI	90 bp
M_INS_B 3'	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA	55 bp	55.86	SpeI, PstI	90 bp
RcsC 5'	GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG	51 bp	55.92	EcoRI, XbaI	1396 bp
RcsC 3'-2	AAGTATACCAACGACTCTTCCATCTTCAC	29 bp	59.63	NdeI	1396 bp	Two extra adenosine was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2]	tgccacctgacgtctaagaa	20 bp	59.44			Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR]	attaccgcctttgagtgagc	20 bp	59.34			Reverse sequencing primer.

@@ Line 3: / Line 3: @@
 !Sequence
 !Length
-!Tm
+!Tm (<math>\[
+^\user1{O} \user1{C}
+\]
+</math>)
 !Restriction site
 !Target length