NYMU Taipei/Primer List
From 2007.igem.org
(Difference between revisions)
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Lihsiangyen (Talk | contribs) |
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| Line 13: | Line 13: | ||
|56.85 | |56.85 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
| - | | | + | |119 bp |
|Potential dimer formation! | |Potential dimer formation! | ||
|- | |- | ||
| Line 22: | Line 22: | ||
|54.74 | |54.74 | ||
|SpeI, PstI | |SpeI, PstI | ||
| - | | | + | |119 bp |
|Potential dimer formation! Stop codons are removed to enable fusion with export signal. | |Potential dimer formation! Stop codons are removed to enable fusion with export signal. | ||
|- | |- | ||
| Line 30: | Line 30: | ||
|60.85 | |60.85 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
| - | | | + | |149 bp |
|Potential dimer formation! | |Potential dimer formation! | ||
|- | |- | ||
| Line 39: | Line 39: | ||
|55.86 | |55.86 | ||
|SpeI, PstI | |SpeI, PstI | ||
| - | | | + | |149 bp |
|Potential dimer formation! Stop codons are removed to enable fusion with export signal. | |Potential dimer formation! Stop codons are removed to enable fusion with export signal. | ||
|- | |- | ||
| Line 48: | Line 48: | ||
|62.45 | |62.45 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
| - | | | + | |122 bp |
|Potential dimer formation! | |Potential dimer formation! | ||
|- | |- | ||
| Line 57: | Line 57: | ||
|57.77 | |57.77 | ||
|SpeI, PstI | |SpeI, PstI | ||
| - | | | + | |122 bp |
|Potential dimer formation! Stop codons are removed to enable fusion with export signal. | |Potential dimer formation! Stop codons are removed to enable fusion with export signal. | ||
|- | |- | ||
| Line 65: | Line 65: | ||
|57.98 | |57.98 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
| - | | | + | |149 bp |
|Potential dimer formation! | |Potential dimer formation! | ||
|- | |- | ||
| Line 74: | Line 74: | ||
|55.10 | |55.10 | ||
|SpeI, PstI | |SpeI, PstI | ||
| - | | | + | |149 bp |
|Stop codons are removed to enable fusion with export signal. | |Stop codons are removed to enable fusion with export signal. | ||
|- | |- | ||
Revision as of 07:10, 23 August 2007
| Name | Sequence | Length | Tm (C) | Restriction site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG | 51 bp | 56.85 | EcoRI, XbaI | 119 bp | Potential dimer formation! |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 119 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| M_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT | 52 bp | 60.85 | EcoRI, XbaI | 149 bp | Potential dimer formation! |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 149 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTGGAACAATGCTG | 51 bp | 62.45 | EcoRI, XbaI | 122 bp | Potential dimer formation! |
| H_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 122 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTGAACCAACACCTGTG | 51 bp | 57.98 | EcoRI, XbaI | 149 bp | Potential dimer formation! |
| H_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 149 bp | Stop codons are removed to enable fusion with export signal. |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1404 bp | |
| RcsC 3'-2 | GGGGTATACCAACGACTCTTCCATCTTCAC | 29 bp | 59.63 | NdeI | 1404 bp | Three extra guanosine was added to the 5' side of the NdeI cutting site. |
| [http://partsregistry.org/Part:BBa_G00100 VF2] | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| [http://partsregistry.org/Part:BBa_G00101 VR] | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
- [http://www.mdbio.com.tw/pfu.htm pfu primer]
