NYMU Taipei/Lab Notes/2007 8 24

From 2007.igem.org

(Difference between revisions)
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Plasmid check
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==Plasmid check==
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3ul DNA+1ul RE
BBa_R0082 psB1A2_pOmpC<br>
BBa_R0082 psB1A2_pOmpC<br>
BBa_I15017 EYFP<br>
BBa_I15017 EYFP<br>
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<p>
<p>
[[Image:NYMU_Taipei_gel_20070824_plasmid_check_1.jpg|300px]]
[[Image:NYMU_Taipei_gel_20070824_plasmid_check_1.jpg|300px]]
 +
*Well 1 OmpR EcoRI
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*Well 2 OmpR SpeI
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*Well 3 OmpR XbaI, PstI
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*Well 4 EYFP EcoRI
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*Well 5 EYFP SpeI
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*Well 6 EYFP XbaI, PstI
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*Well 7 100bp ladder
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*Well 8 ECFP EcoRI
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*Well 9 ECFP SpeI
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*Well 10 ECFP XbaI, PstI
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*Well 11 HSL EcoRI
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*Well 12 HSL SpeI
</p>
</p>
<p>
<p>
[[Image:NYMU_Taipei_Gel_20070824_plasmid_check_2.jpg|300px]]
[[Image:NYMU_Taipei_Gel_20070824_plasmid_check_2.jpg|300px]]
 +
</p>
</p>

Revision as of 09:02, 24 August 2007

Plasmid check

3ul DNA+1ul RE BBa_R0082 psB1A2_pOmpC
BBa_I15017 EYFP
BBa_I15016 ECFP
BBa_C0076 HSL
BBa_B0034 RBS
BBa_C0077 CinR
BBa_B0014 D-term
BBa_R0077 pCinRHSL RBS+
BBa_R0078 pCinRHSL

NYMU Taipei gel 20070824 plasmid check 1.jpg

  • Well 1 OmpR EcoRI
  • Well 2 OmpR SpeI
  • Well 3 OmpR XbaI, PstI
  • Well 4 EYFP EcoRI
  • Well 5 EYFP SpeI
  • Well 6 EYFP XbaI, PstI
  • Well 7 100bp ladder
  • Well 8 ECFP EcoRI
  • Well 9 ECFP SpeI
  • Well 10 ECFP XbaI, PstI
  • Well 11 HSL EcoRI
  • Well 12 HSL SpeI

NYMU Taipei Gel 20070824 plasmid check 2.jpg