From 2007.igem.org

Revision as of 09:22, 24 August 2007 (view source) Naldo (Talk | contribs) (→Plasmid check) ← Older edit		Revision as of 10:55, 24 August 2007 (view source) Lihsiangyen (Talk | contribs) (→Plasmid check) Newer edit →
Line 1:		Line 1:
	==Plasmid check==		==Plasmid check==
-	3ul DNA+1ul RE, final volume: 20ul<br>	+	3ul DNA (5ml LB E. coli, miniprep in 50ul EB)+1ul RE, final volume: 20ul<br>
	BBa_R0082 psB1A2_pOmpC<br>		BBa_R0082 psB1A2_pOmpC<br>
	BBa_I15017 EYFP<br>		BBa_I15017 EYFP<br>

---

Revision as of 10:55, 24 August 2007

Plasmid check

3ul DNA (5ml LB E. coli, miniprep in 50ul EB)+1ul RE, final volume: 20ul
BBa_R0082 psB1A2_pOmpC
BBa_I15017 EYFP
BBa_I15016 ECFP
BBa_C0076 HSL
BBa_B0034 RBS
BBa_C0077 CinR
BBa_B0014 D-term
BBa_R0077 pCinRHSL RBS+
BBa_R0078 pCinRHSL

• Well 1 OmpR EcoRI
• Well 2 OmpR SpeI
• Well 3 OmpR XbaI, PstI
• Well 4 EYFP EcoRI
• Well 5 EYFP SpeI
• Well 6 EYFP XbaI, PstI
• Well 7 100bp ladder
• Well 8 ECFP EcoRI
• Well 9 ECFP SpeI
• Well 10 ECFP XbaI, PstI
• Well 11 HSL EcoRI
• Well 12 HSL SpeI

• Well 1 HSL XbaI, PstI
• Well 2 CinR EcoRI
• Well 3 CinR SpeI
• Well 4 CinR XbaI, PstI
• Well 5 pCinRHSL EcoRI
• Well 6 pCinRHSL SpeI
• Well 7 100bp ladder
• Well 8 pCinRHSL XbaI, PstI
• Well 9 Empty
• Well 10 pCinRHSL+RBS EcoRI
• Well 11 pCinRHSL+RBS SpeI
• Well 12 pCinRHSL+RBS XbaI, PstI

• Well 1 Empty
• Well 2 RBS EcoRI
• Well 3 RBS SpeI
• Well 4 RBS XP
• Well 5 100bp ladder
• Well 6 D-term EcoRI
• Well 7 Leaky well
• Well 8 D-term EcoRI
• Well 9 D-term XbaI, PstI