Week 5
From 2007.igem.org
(Difference between revisions)
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::'''07/31/07''' | ::'''07/31/07''' | ||
| + | *There isn’t any colony for C0012, so we inoculate I52034 and B0015 colony in 5 ml. | ||
| + | *We plate 30/07 ligations. | ||
| + | *We get along with the J04431 fluorescence test: | ||
| + | -From the glycerol stock we sprout 1 ml at 37°C for 1 hour in agitation. | ||
| + | -We verify the OD and the fluorescence every 15 minutes. The fluorescence compare at the istance t=210min and at the OD=0.5. | ||
| + | *At the end of this test we decide to repeat it to see if we can switch off the fluorescent cells with a major quantity of glucose to confirm our assumptions. | ||
| + | We will attend this test on 07/08. | ||
Revision as of 12:17, 27 August 2007
- 07/30/07
- We control bacterial grew (link).
- Transformation for C0012 and J52034 and B0015.
- We get along with the ligation R0051+I13507 (I763007) (vector 2 ul, insert 6 ul) and the ligation J04500+ C0051 (I763005) (vector 2 ul, insert 10 ul).
- 07/31/07
- There isn’t any colony for C0012, so we inoculate I52034 and B0015 colony in 5 ml.
- We plate 30/07 ligations.
- We get along with the J04431 fluorescence test:
-From the glycerol stock we sprout 1 ml at 37°C for 1 hour in agitation. -We verify the OD and the fluorescence every 15 minutes. The fluorescence compare at the istance t=210min and at the OD=0.5.
- At the end of this test we decide to repeat it to see if we can switch off the fluorescent cells with a major quantity of glucose to confirm our assumptions.
We will attend this test on 07/08.
- 08/01/07
- 08/02/07
- 08/03/07
