McGill
From 2007.igem.org
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| + | <center>This is the official wiki of the McGill University iGEM 2007 team from Montreal, Quebec, Canada</center><br> | ||
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== Project Overviews == | == Project Overviews == | ||
<u>'''Team 1 - Fluorescence Complimentation'''</U> | <u>'''Team 1 - Fluorescence Complimentation'''</U> | ||
| - | This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that | + | This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that reversibly associate.<br> |
[[McGill/Team_1_More_Information|More Information]] <br> | [[McGill/Team_1_More_Information|More Information]] <br> | ||
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| - | Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]<br> | + | <center>Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]</center><br> |
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== In the Lab == | == In the Lab == | ||
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'''<nowiki>[[McGill/Team_1:_Fluorescence_Complementation|Team 1: Fluorescence Complementation]]</nowiki>''' | '''<nowiki>[[McGill/Team_1:_Fluorescence_Complementation|Team 1: Fluorescence Complementation]]</nowiki>''' | ||
| - | Thanks! - '''Hanmo''' | + | Thanks! - '''Hanmo'''__NOTOC__ |
Revision as of 16:33, 19 May 2007
Project OverviewsTeam 1 - Fluorescence Complimentation This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence. We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence. These half proteins will then be attached to proteins that reversibly associate. More Information
Our project consists of a two-gene oscillator with coupling by an Autoinducer (AI). This system was presented last year. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations at different cell densities. More Information
In the LabThe TeamImportant NotesWhen you make the links for the wiki pages, please follow this example: [[McGill/Link_to_page|Display Name]] An actual link would look like this in wiki code: [[McGill/Team_1:_Fluorescence_Complementation|Team 1: Fluorescence Complementation]] Thanks! - Hanmo |
