From 2007.igem.org

Revision as of 05:20, 30 August 2007 (view source) Lihsiangyen (Talk | contribs) ← Older edit		Revision as of 05:21, 30 August 2007 (view source) Lihsiangyen (Talk | contribs) Newer edit →
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	*Enzyme Digestion (RBS)		*Enzyme Digestion (RBS)
-	**pOmpC: 11ul	+	**RBS: 11ul
	**SpeI: 1ul		**SpeI: 1ul
	**PstI: 1ul		**PstI: 1ul

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Revision as of 05:21, 30 August 2007

• Enzyme Digestion (RBS)
  • RBS: 11ul
  • SpeI: 1ul
  • PstI: 1ul
  • 10x buffer: 2, 2ul
  • 10x BSA: 2ul
  • H2O: 3ul
  • Total: 20ul
  • 37°C, 2h
  • Heat inactivation: 65°C 20min
• Enzyme Digestion (cinR)
  • cinR: 11ul
  • XbaI: 1ul
  • PstI: 1ul
  • 10x buffer: 3, 2ul
  • 10x BSA: 2ul
  • H2O: 3ul
  • Total: 20ul
  • 37°C, 2h
  • Heat inactivation: 65°C 20min
• Enzyme Digestion (HSL)
  • HSL: 4ul
  • XbaI: 1ul
  • PstI: 1ul
  • 10x buffer: 3, 2ul
  • 10x BSA: 2ul
  • H2O: 10ul
  • Total: 20ul
  • 37°C, 2h
  • Heat inactivation: 65°C 20min