NYMU Taipei/Lab Notes/2007 8 30
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**37°C, 2h | **37°C, 2h | ||
**Heat inactivation: 65°C 20min | **Heat inactivation: 65°C 20min | ||
| + | *Plasmid extraction: H_IGFBP3, H_IGFBP7, H_INS, M_IDE | ||
| + | **From 5ml LB medium to 50ul EB | ||
Revision as of 06:36, 30 August 2007
- Enzyme Digestion (RBS)
- RBS: 11ul
- SpeI: 1ul
- PstI: 1ul
- 10x buffer: 2, 2ul
- 10x BSA: 2ul
- H2O: 3ul
- Total: 20ul
- 37°C, 2h
- Heat inactivation: 65°C 20min
- Enzyme Digestion (cinR)
- cinR: 11ul
- XbaI: 1ul
- PstI: 1ul
- 10x buffer: 3, 2ul
- 10x BSA: 2ul
- H2O: 3ul
- Total: 20ul
- 37°C, 2h
- Heat inactivation: 65°C 20min
- Enzyme Digestion (HSL)
- HSL: 4ul
- XbaI: 1ul
- PstI: 1ul
- 10x buffer: 3, 2ul
- 10x BSA: 2ul
- H2O: 10ul
- Total: 20ul
- 37°C, 2h
- Heat inactivation: 65°C 20min
- Plasmid extraction: H_IGFBP3, H_IGFBP7, H_INS, M_IDE
- From 5ml LB medium to 50ul EB
