NYMU Taipei/Lab Notes/2007 9 1
From 2007.igem.org
(Difference between revisions)
Lihsiangyen (Talk | contribs) |
Lihsiangyen (Talk | contribs) |
||
| Line 1: | Line 1: | ||
| - | *Plasmid extraction: RBS+cinR 1, RBS+HSL 1, RBS+HSL 2, RBS+HSL 3 | + | *Plasmid extraction: RBS+cinR 1, RBS+HSL 1, RBS+HSL 2, RBS+HSL 3 from 5ml LB culture to 50ul EB |
*The concentration was measured with spectrophotometer at 260nm | *The concentration was measured with spectrophotometer at 260nm | ||
**RBS+cinR 1: 8ug/ul | **RBS+cinR 1: 8ug/ul | ||
Revision as of 08:27, 1 September 2007
- Plasmid extraction: RBS+cinR 1, RBS+HSL 1, RBS+HSL 2, RBS+HSL 3 from 5ml LB culture to 50ul EB
- The concentration was measured with spectrophotometer at 260nm
- RBS+cinR 1: 8ug/ul
- RBS+HSL 1: 3ug/ul
- RBS+HSL 2: 8ug/ul
- RBS+HSL 3: 6ug/ul
- Enzyme digest RBS+cinR 1
- RBS+cinR 1: 14ul
- SpeI: 1ul
- PstI 1ul
- 10x buffer 2: 2ul
- 10x BSA: 2ul
- 37°C water bath, 2h
- Heat inactivation: 65°C, 20min
- Enzyme digest RBS+HSL 2
- RBS+HSL 2: 14ul
- XbaI: 1ul
- PstI: 1ul
- 10x buffer 3: 2ul
- 10x BSA: 2ul
- 37°C water bath, 2h
- Heat inactivation: 65°C, 20min
- Enzyme digest RBS+HSL 3
- RBS+HSL 3: 14ul
- XbaI: 1ul
- PstI: 1ul
- 10x buffer 3: 2ul
- 10x BSA: 2ul
- 37°C water bath, 2h
- Heat inactivation: 65°C, 20min
- Check RBS+cinR 1 with BsaBI digestion
- RBS+cinR 1: 3ul
- BsaBI: 1ul
- 10x buffer 2: 2ul
- H2O: 14ul
- 60°C dry bath, 1h
