BerkiGEM2007-AustinConstructionFile64
From 2007.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
Digest I716356 (BamHI/Xho, 4008/9, Larger) <br> | Digest I716356 (BamHI/Xho, 4008/9, Larger) <br> | ||
Ligation product is I716364<br> | Ligation product is I716364<br> | ||
| + | |||
| + | |||
| + | <br><br> | ||
| + | Dbbs Method: | ||
| + | PCR 1099R/ca998 on I716269 (4032bp, BglII, BsaI, DpnI)<br> | ||
| + | PCR 1099F/g00101 on I716354 (3151bp, BamHI, BsaI, DpnI)<br> | ||
| + | Ligation product is I716362 (6843 bp)<br> | ||
Revision as of 21:24, 10 September 2007
Digest I716269 (BglII/XhoI/ScaI, 2835/1504/559, largest)
Digest I716356 (BamHI/Xho, 4008/9, Larger)
Ligation product is I716364
Dbbs Method:
PCR 1099R/ca998 on I716269 (4032bp, BglII, BsaI, DpnI)
PCR 1099F/g00101 on I716354 (3151bp, BamHI, BsaI, DpnI)
Ligation product is I716362 (6843 bp)
