Week 10
From 2007.igem.org
| Line 21: | Line 21: | ||
-B0015 with Eco/Xba; | -B0015 with Eco/Xba; | ||
| - | *Band | + | *Band extraction; |
*Ligation for: | *Ligation for: | ||
| Line 86: | Line 86: | ||
*New control digestion for: | *New control digestion for: | ||
| - | -I763019 with | + | -I763019 with Xba/Spe; |
| + | |||
| + | -I763019 with Xba/Pst1; | ||
-I763021 with Eco/Spe; | -I763021 with Eco/Spe; | ||
| Line 93: | Line 95: | ||
-I763030 with Eco/Spe; | -I763030 with Eco/Spe; | ||
| + | |||
| + | *Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026; | ||
| + | *Ligation O/N for: | ||
| + | |||
| + | -I763026+I763019(I763028); | ||
| + | |||
| + | -I763026+I763004(I763027); | ||
Revision as of 20:08, 7 September 2007
- 09/03/07
- Miniprep for:
-R0040+S0100;
-R0040+S03520;
-I763005+I763015;
-J52034+E0022;
- Digestion for:
-R0040+S0100 with Eco/Spe;
-R0040+S03520 with Eco/Spe;
-I763005+I763015 with Spe/Pst1;
-J52034+E0022with Spe/Pst1;
-B0015 with Eco/Xba;
- Band extraction;
- Ligation for:
-I763025+B0015(I763026);
-I763024+B0015(I763030);
-I763016+I763020(I763019);
-I763009+P0412(I763021);
-I763009+S0100(I763023);
-I763009+S03520(I763022);
-R0051+I763020(I763011);
- 09/04/07
- There are colonies for every ligations.
- 09/05/07
Miniprep for:
-I763016+I763020(I763019);
-I763025+B0015(I763026);
-I763024+B0015(I763030);
-R0051+I763020(I763011);
-I763009+P0412(I763021);
-I763009+S03520(I763022);
- Digestion for:
-I763019 with Eco/Xba;
-I763026 with Eco/Spe;
-I763030 with Eco/Spe;
-I763011 with Xba/Pst1;
-R0051+I13507(I763007) with Xba/Pst1;
-I763021 with Eco/Spe;
- Control digestion for I763019 with Eco/Pst1;
- Band extraction for
-I763019;
-I763026;
-I763011;
-R0051+I13507(I763007);
- New control digestion for:
-I763019 with Xba/Spe;
-I763019 with Xba/Pst1;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe;
-I763030 with Eco/Spe;
- Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026;
- Ligation O/N for:
-I763026+I763019(I763028);
-I763026+I763004(I763027);
- 09/06/07
- 09/07/07
