From 2007.igem.org

Revision as of 10:20, 10 September 2007 (view source) Lihsiangyen (Talk | contribs) ← Older edit		Revision as of 10:23, 10 September 2007 (view source) Lihsiangyen (Talk | contribs) Newer edit →
Line 10:		Line 10:
	**compenent cell:100ul		**compenent cell:100ul
	*apply the cells on the agar plate		*apply the cells on the agar plate
		+
		+	*plate culture failed!
		+	*possibility:concentration of H_INS_A1 is not high enough

---

Revision as of 10:23, 10 September 2007

• Ligation(H_INS_A)
  • H_INS_A1:1ul
  • pOmpC:2ul
  • 10X ligase:1 ul
  • 10X buffer:1 ul
  • ddH2O:5ul
  • total: 10ul
• Transformation
  • ligated DNA:2ul
  • compenent cell:100ul
• apply the cells on the agar plate

• plate culture failed!
• possibility:concentration of H_INS_A1 is not high enough