NYMU Taipei/Lab Notes/2007 9 11
From 2007.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
*** insert 300 ng | *** insert 300 ng | ||
*** ligase 1uL | *** ligase 1uL | ||
| - | + | *** add water to make total volume as 10 uL | |
| - | *** total 10 uL | + | |
Revision as of 14:50, 11 September 2007
- Fresh back
- two different PCR (INS_A and INS_B) have been done by 海威 (9/5, 2007) and 惇茹 (9/8, 2007)
- we had failed three times for construction of pOmpC + INS_A using 惇茹's INS_A and following three different ligation protocol
- insert (1 uL) vs. vector (1 uL) at 09/09, 2007
- insert (2 uL) vs. vector (1 uL) at 09/10, 2007
- insert (3 uL) vs. vector (1 uL) at 09/10, 2007
- a trivial failure (absent of vector part) at 09/08, 2007
- According to YEA ligation protocol, it requires
- ligation buffer A 1uL
- ligation buffer B 1uL
- vector 50 ng or 100 ng (proposed by pett)
- insert 300 ng
- ligase 1uL
- add water to make total volume as 10 uL
