NYMU Taipei/Lab Notes/2007 9 13

From 2007.igem.org

(Difference between revisions)
Line 7: Line 7:
*** [[Image:20070913-INS A imageJ.jpg]]
*** [[Image:20070913-INS A imageJ.jpg]]
*** marker (100 bp, 40ng/10uL = 4ng/uL)
*** marker (100 bp, 40ng/10uL = 4ng/uL)
 +
*** the best temperature should be around 55.5 degree
 +
* PCR product concentration summary
 +
{|
 +
!name
 +
!product
 +
!dulition
 +
!A260
 +
!A260/A280
 +
!A260/A230
 +
|-
 +
|9/5 海威
 +
|INS_A #1 (A1)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|-
 +
|9/5 海威
 +
|INS_A #2 (A2)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|-
 +
|9/5 海威
 +
|INS_B #1 (B1)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|-
 +
|9/5 海威
 +
|INS_B #2 (B2)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|-
 +
|9/12 德銘
 +
|INS_A #1 (A1, 49.8)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|-
 +
|9/12 德銘
 +
|INS_A #2 (A2, 52.3)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|-
 +
|9/12 德銘
 +
|INS_A #3 (A3, 55.5)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|-
 +
|9/12 德銘
 +
|INS_A #4 (A4, 58.7)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|-
 +
|9/12 德銘
 +
|INS_A #5 (A1, 60.9)
 +
|6uL + 594uL ddH2O
 +
|
 +
|
 +
|
 +
|}

Revision as of 13:08, 13 September 2007

  • PCR issue
    • We tested our PCR product (5uL) from different annealing temperature via GEL
    • From imageJ comparison, the concentration of product rise with temperature before 60 degree
      • GEL (left to right: 49.8, 52.3, 55.5, 58.7, 60.9 degree)
      • 20070913-INS A GEL.jpg
      • imageJ
      • 20070913-INS A imageJ.jpg
      • marker (100 bp, 40ng/10uL = 4ng/uL)
      • the best temperature should be around 55.5 degree
  • PCR product concentration summary
name product dulition A260 A260/A280 A260/A230
9/5 海威 INS_A #1 (A1) 6uL + 594uL ddH2O
9/5 海威 INS_A #2 (A2) 6uL + 594uL ddH2O
9/5 海威 INS_B #1 (B1) 6uL + 594uL ddH2O
9/5 海威 INS_B #2 (B2) 6uL + 594uL ddH2O
9/12 德銘 INS_A #1 (A1, 49.8) 6uL + 594uL ddH2O
9/12 德銘 INS_A #2 (A2, 52.3) 6uL + 594uL ddH2O
9/12 德銘 INS_A #3 (A3, 55.5) 6uL + 594uL ddH2O
9/12 德銘 INS_A #4 (A4, 58.7) 6uL + 594uL ddH2O
9/12 德銘 INS_A #5 (A1, 60.9) 6uL + 594uL ddH2O
Retrieved from "http://2007.igem.org/wiki/index.php/NYMU_Taipei/Lab_Notes/2007_9_13"