User:Lihsiangyen

From 2007.igem.org

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<h2>Personal schedule</h2>
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<h3>Aug 2007</h3>
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<p>
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<table width="50%" border="1" style="text-align: left;">
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    <tbody>
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        <tr>
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            <td style="text-align: center; font-weight: bold; background-color: rgb(204, 255, 204);"><big>Sun.</big></td>
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            <td style="text-align: center; font-weight: bold; background-color: rgb(204, 255, 204);"><big>Mon.</big></td>
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            <td style="text-align: center; font-weight: bold; background-color: rgb(204, 255, 204);"><big>Tue.</big></td>
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            <td style="text-align: center; font-weight: bold; background-color: rgb(204, 255, 204);"><big>Wed.</big></td>
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            <td style="text-align: center; font-weight: bold; background-color: rgb(204, 255, 204);"><big>Thr.</big></td>
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            <td style="text-align: center; font-weight: bold; background-color: rgb(204, 255, 204);"><big>Fri.</big></td>
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            <td style="text-align: center; font-weight: bold; background-color: rgb(204, 255, 204);"><big>Sat.</big></td>
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        </tr>
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        <tr>
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            <td align="center">&nbsp;</td>
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            <td align="center">&nbsp;</td>
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            <td align="center">&nbsp;</td>
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            <td align="center">1</td>
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            <td align="center">2</td>
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            <td align="center">3</td>
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            <td align="center">4</td>
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        </tr>
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            <td align="center">5</td>
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            <td align="center">6</td>
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            <td align="center">7</td>
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            <td align="center">8</td>
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            <td align="center">9</td>
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            <td align="center">10</td>
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            <td align="center">11</td>
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        </tr>
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            <td align="center">12</td>
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            <td align="center">13</td>
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            <td align="center">14</td>
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            <td align="center">15</td>
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            <td align="center">16</td>
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            <td align="center">17</td>
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            <td align="center">18</td>
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        </tr>
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            <td align="center">19</td>
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            <td align="center">20</td>
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            <td align="center">21</td>
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            <td align="center">22</td>
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            <td align="center">23</td>
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            <td align="center">24</td>
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            <td align="center">25</td>
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        </tr>
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        <tr>
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            <td align="center">26</td>
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            <td align="center">27</td>
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            <td align="center">28</td>
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            <td align="center">29</td>
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            <td align="center">30</td>
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            <td align="center">31</td>
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            <td align="center">&nbsp;</td>
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        </tr>
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    </tbody>
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</table>
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</p>
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<h4>Aug 6</h4>
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<ul>
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    <li>Determine the total amount of lab work required</li>
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    <li>Find all the available relevent protocol in Dr. Chang's lab</li>
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    <li>Write down the schedule and deadline in August</li>
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    <li>Divid the big project into several small project</li>
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</ul>
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<h4>Aug 7</h4>
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<ul>
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    <li>Allocate each small project to different people</li>
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    <li>Set small project deadline</li>
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    <li>Start phase 1 project</li>
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    <li>Determine a regular meeting time for lab work core member</li>
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</ul>
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<h4>&nbsp;Aug 10</h4>
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<p>Useful information:</p>
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<ul>
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    <li>Amp stock conc.: 50mg/ml</li>
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    <li>Amp working conc,: 50ug/ml</li>
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    <li>Kan stock conc.: 50mg/ml</li>
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    <li>Kan working conc.: 20~30ug/ml</li>
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    <li>Liquid medium: 5ug/ml</li>
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    <li>It is difficult to clone DNA segments longer than about 15,000 bp when plasmid are used as the vector.</li>
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</ul>
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<h4>Aug 11</h4>
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<ul>
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    <li>EnvZ information: <a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=48994873&amp;db=Nucleotide&amp;from=3532538&amp;to=3533890&amp;view=gbwithparts">DNA sequence</a> and <a href="http://www.pir.uniprot.org/cgi-bin/upEntry?id=P0AEJ4">protein domain prediction</a></li>
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    <li>Basic design:</li>
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</ul>
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<p>[[Image:RcsC_EnvZ_design.jpg|400px]]</p>
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<h4>Aug 15</h4>
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<p>Useful information:</p>
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<ul>
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    <li><font size="2">[[Planning]] </font></li>
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    <li><font size="2">[[Planning-New]]</font></li>
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    <li><a href="http://openwetware.org/wiki/Endy:M9_media/supplemented"><font size="2">Glucose-free M9 media</font></a></li>
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    <li><font size="2">[[Ribosome binding sites information]]</font></li>
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    <li><a href="http://partsregistry.org/partsdb/pgroup.cgi?pgroup=terminator"><font size="2">Transcriptional terminator</font></a></li>
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    <li><a href="http://partsregistry.org/Featured_Parts:Cell_Death"><font size="2">Cell death gene as a construction tool</font></a><font size="2"> </font></li>
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    <li><a href="http://partsregistry.org/partsdb/pgroup.cgi?pgroup=Plasmid"><font size="2">Available vectors</font></a></li>
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    <li><a href="http://partsregistry.org/Part:BBa_V1005">DB3.1</a></li>
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    <li><font size="2">[[Available reporter fragment]]</font></li>
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    <li><a href="http://partsregistry.org/partsdb/pgroup.cgi?pgroup=Regulatory"><font size="2">Available promotor</font></a></li>
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    <li><a href="http://partsregistry.org/Part:BBa_G00100">VF2</a> &amp; <a href="http://partsregistry.org/Part:BBa_G00101">VR</a></li>
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    <li><a href="http://partsregistry.org/Part_Types:Measurement_Systems">measurement system</a></li>
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    <li><a href="http://partsregistry.org/cgi/htdocs/Assembly/restriction_enzymes.cgi">Standard assembly</a></li>
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</ul>
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<p>cinR &amp; OmpC dual controlled promotor for IDE, IGFBP3 and IGFBP7</p>
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<ul>
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    <li>
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    <div><a href="http://partsregistry.org/Part:BBa_R0077">Part:BBa_R0077:</a> &nbsp;&nbsp;&nbsp; 17L&nbsp;&nbsp; &nbsp;iGEM 2007 Parts Kit Plate 1&nbsp;&nbsp; &nbsp;pSB1A2&nbsp;&nbsp;&nbsp;&nbsp; 231 bp (with RBS)</div>
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    </li>
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    <li><a href="http://partsregistry.org/Part:BBa_C0078">Part:BBa_R0078</a>:&nbsp;&nbsp;&nbsp;&nbsp; 15D&nbsp;&nbsp;&nbsp; iGEM 2007 Parts Kit Plate 1&nbsp;&nbsp;&nbsp; pSB1A2&nbsp;&nbsp;&nbsp;&nbsp; 225 bp (without RBS)</li>
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    <li><a href="http://partsregistry.org/Part:BBa_C0077">Part:BBa_C0077</a>: &nbsp;&nbsp;&nbsp; 7G&nbsp;&nbsp; &nbsp; &nbsp; iGEM 2007 Parts Kit Plate 2&nbsp;&nbsp; &nbsp;pSB2K3&nbsp;&nbsp;&nbsp;&nbsp; 762 bp</li>
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    <li><a href="http://partsregistry.org/Part:BBa_C0076">Part:BBa_C0076</a>:&nbsp;&nbsp;&nbsp;&nbsp; 7E&nbsp;&nbsp; &nbsp; &nbsp; iGEM 2007 Parts Kit Plate 2&nbsp;&nbsp;&nbsp; pSB2K3&nbsp;&nbsp;&nbsp;&nbsp; 702 bp</li>
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</ul>
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<h4>Aug 20</h4>
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<ul>
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    <li>重新設計primer: 5'端3~5個GC, 避免與template annealing。</li>
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</ul>
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<h4>Aug 24</h4>
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<ul>
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    <li>Check plasmid時找一個切在insert裡的RE check</li>
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    <li>Plasmid有排他性，不一定可以把兩個plasmid同時塞在同一隻細菌裡。</li>
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    <li>解決：E. coli會intake glucose的問題。</li>
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</ul>
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<h3>Sep 2007</h3>
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<h4>Sep 14</h4>
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<ul>
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    <li>Putative OmpRBS sequence: tacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgt</li>
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    <li>&nbsp;pOmpC domain: [[Image:POmpC_domain.png]]</li>
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</ul>
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Latest revision as of 13:15, 16 September 2007

Retrieved from "http://2007.igem.org/wiki/index.php/User:Lihsiangyen"