Bangalore

From 2007.igem.org

Revision as of 07:31, 29 May 2007

The Company

• Mukund: Chief Mentor, Consultant and The Godfather
• Sugat: Right-hand Man and Manager of Operations
• Nilesh: The Man for all Seasons
• Vivek: Chief Troubleshooter, often his role is confused with one of a Troublemaker
• Krishna: The Sharpshooter
• Senthil: The Wikiman, also fondly referred to as the "Bio Info God"
• Varun: The Voice of Reason and Anti-Reason
• K12Z1: The Workhorse

The Mission

To investigate multistability and hysteresis in combinatorially constructed synthetic Vibrio quorum sensing circuits

The Target - Vibrio Quorum Sensing System

Quorum sensing is a phenomenon by which bacteria sense a critical cell density before turning on the expression of certain genes. It involves the gradual build-up of a chemical termed the 'autoinducer' in the cell. The autoinducer freely diffuses across the cell membrane and hence, its concentration is population density dependent. When the concentration crosses a threshold, the bacteria switch to a different physiological state such as bioluminescence, virulent gene expression, and bio-film formation.

In Vibrio fischeri, when the population density (and hence the concentration of autoinducer) crosses a certain threshold, the expression of a set of genes that is required for bioluminescence is turned on. The production of the autoinducer is under the control of a gene, the expression of which involves positive feedback. The following figure shows the various components of this system (Ref. 1).