Edinburgh/DivisionPopper/Status

From 2007.igem.org

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__TOC__
__TOC__
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==Status==
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==Lab details==
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{|style="width:95%; background-color:#fcaf3e;" cellpadding="10"
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In this section we explain all the details of the lab work (host used, plasmid used, problems encountered etc..)
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!Part || For use in || Current Status
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(please Caro update this section).
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|-
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|Dif sites || Exp1, Exp2, Final PoPper || Done and ready to be tested
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==Biobricks==
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|-
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|Reversed Promoter B (LacI)|| Exp1, Exp3, Final PoPper || Done
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For the construction of experiments and the Division PoPper several Biobricks are needed. Some of them have been taken from the Registry of Standard Parts and some have been constructed appositely. The table below presents the list and their current status in the lab:
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(please Caro update this section with the other biobricks).
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{| border="1" cellpadding="20" cellspacing="0"
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|-
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|Reporter || Exp1, Exp2, Exp3|| Revived and transformed [http://partsregistry.org/Part:BBa_E0422 BBa_E0422] but it refuses to show up on gels. Currently being sequenced. YFP is our backup; it is revived, sequenced and ready to go.
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|Biobrick
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|Used in
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|Status
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|Registry entry
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|-
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|Inverter (CI Lambda)|| Exp3 || revived [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]. Another part that, once PCR'd, refuses to show up on the gel. Being sequenced.
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|Forward dif site
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|Exp1, Exp2, Division PoPper
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|Constructed and Registered
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|[http://partsregistry.org/Part:BBa_I742101 BBa_I742101]
|-
|-
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|Promoter 0 || Exp3, Final PoPper|| being designed
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|Bacwardk dif site
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|Exp1,Exp2,Division PoPper
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|Constructed and Registered
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|[http://partsregistry.org/Part:BBa_I742102 BBa_I742102]
|-
|-
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|Promoter A || Final PoPper|| being designed
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|x
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|x
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|x
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|x
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|-
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|Repressor (+RBS) A || Final PoPper|| being designed
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|x
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|x
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|x
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|x
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|-
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|Reversed Repressor (+RBS)B || Final PoPper || Not started
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|x
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|x
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|x
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|x
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|-
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|Central Terminator || Final PoPper|| designed
 
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Revision as of 13:13, 22 September 2007

MENU : Introduction | Background | Applications | Design | Modelling | Status | Conclusions

Contents


Lab details

In this section we explain all the details of the lab work (host used, plasmid used, problems encountered etc..) (please Caro update this section).

Biobricks

For the construction of experiments and the Division PoPper several Biobricks are needed. Some of them have been taken from the Registry of Standard Parts and some have been constructed appositely. The table below presents the list and their current status in the lab: (please Caro update this section with the other biobricks).

Biobrick Used in Status Registry entry
Forward dif site Exp1, Exp2, Division PoPper Constructed and Registered [http://partsregistry.org/Part:BBa_I742101 BBa_I742101]
Bacwardk dif site Exp1,Exp2,Division PoPper Constructed and Registered [http://partsregistry.org/Part:BBa_I742102 BBa_I742102]
x x x x
x x x x
x x x x