Biobrick Restriction Enzyme
From 2007.igem.org
(Difference between revisions)
Line 11: | Line 11: | ||
3'---CTTAA G---5' | 3'---CTTAA G---5' | ||
|- | |- | ||
- | |[[XbaI|''Xba''I]] | + | |[[XbaI|''Xba''I]]||''[[Xanthomonas badrii]]'' |
| | | | ||
5'TCTAGA | 5'TCTAGA | ||
Line 21: | Line 21: | ||
|colspan=4|* = blunt ends | |colspan=4|* = blunt ends | ||
|- | |- | ||
- | |[[PstI|''Pst''I]] | + | |[[PstI|''Pst''I]]||''[[Providencia stuartii]]'' |
| | | | ||
5'CTGCAG | 5'CTGCAG | ||
Line 28: | Line 28: | ||
5'---CTGCA G---3' | 5'---CTGCA G---3' | ||
3'---G ACGTC---5' | 3'---G ACGTC---5' | ||
+ | |- | ||
+ | |[[SpeI|''SpeI''I]]||''[[]]'' | ||
+ | | | ||
+ | 5' | ||
+ | 3' | ||
+ | | | ||
+ | 5'--- ---3' | ||
+ | 3'--- ---5' | ||
|- | |- | ||
|} | |} |
Revision as of 12:46, 20 September 2007
We have selected and for promoters and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner.
Enzyme | Source | Recognition Sequence | Cut |
---|---|---|---|
EcoRI | Escherichia coli |
5'GAATTC 3'CTTAAG |
5'---G AATTC---3' 3'---CTTAA G---5' |
XbaI | Xanthomonas badrii |
5'TCTAGA 3'AGATCT |
5'---T CTAGA---3' 3'---AGATC T---5' |
* = blunt ends | |||
PstI | Providencia stuartii |
5'CTGCAG 3'GACGTC |
5'---CTGCA G---3' 3'---G ACGTC---5' |
SpeII | [[]] |
5' 3' |
5'--- ---3' 3'--- ---5' |