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	::'''09/17/07'''		::'''09/17/07'''
	*Ligations for:		*Ligations for:
-	-I763026 + I763019;	+	-[http://partsregistry.org/Part:BBa_I763026 I763026] + -[http://partsregistry.org/Part:BBa_I763019 I763019];
-	-I763026 + I763004;	+	-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004];
	-I763025 + J04031.		-I763025 + J04031.

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Revision as of 13:45, 20 September 2007

09/17/07

• Ligations for:

-[http://partsregistry.org/Part:BBa_I763026 I763026] + -[http://partsregistry.org/Part:BBa_I763019 I763019];

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004];

-I763025 + J04031.

• We transform ligations and strake them on plates.

09/18/07

• We inoculate a colony for yesterday ligations in 5ml of LB medium O/N.

09/19/07

• Miniprep for:

-I763027;

-J04431;

-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).

• Digestion for:

-I763027 with Eco/Spe;

-J04431 with Eco/Spe;

-Ptet-LacI-GFP with Eco/Spe;

-I763007 with Eco/Xba;

-Plac-cI-LacY with Eco/Spe1;

• Band extraction from gel for all digestion and then we observe:

-I763027, J04431 are died;

-Ptet-LacI-GFP is correct;

-I763007 not found;

-Plac-cI-LacY is correct.

09/20/07

09/21/07

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