Biobrick Primers Design
From 2007.igem.org
(Difference between revisions)
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| + | [[Image:Primers.jpg]] | ||
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'''''FOX3 XbaI Fw''''' | '''''FOX3 XbaI Fw''''' | ||
| - | 5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’ | + | 5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’ |
Tm 60°C | Tm 60°C | ||
| Line 17: | Line 19: | ||
Tm 60°C | Tm 60°C | ||
| - | '''''Luciferase Spe I Rv'' ''' | + | '''''Luciferase Spe I Rv'' ''' |
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’ | 5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’ | ||
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Tm 56°C | Tm 56°C | ||
| - | |||
Revision as of 13:39, 20 September 2007
FOX3 XbaI Fw
5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
Tm 60°C
'''FOX3 SpeI Rev
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
Tm 50°C
Luciferase Xba I Fv
5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’
Tm 60°C
Luciferase Spe I Rv
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
Tm 60°C
2XORE – CYC1 XbaI Fw
5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
Tm 58,4°C
1X ORE – CYC1 XbaI Fw
5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
Tm 56°C
2&1ORE-CYC1 Spe I Rev
5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
Tm 56°C
