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	::'''10/22/07'''		::'''10/22/07'''
		+	*Ligation:
		+	::-pLac-LacY-GFP on pSB4A3;
		+
		+	*Transformation:
		+	::-pLac-LacY-GFP on pSB4A3;
		+
		+	*We inoculate:
		+	::-pLac-GFP-pTet-LacI (on high copy plasmid);
		+	::-pLac-cI-GFP (on low copy plasmid), 3 different inocules using 3 different colonies;
		+	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).
		+	::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).

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Revision as of 08:47, 23 October 2007

10/22/07

• Ligation:

-pLac-LacY-GFP on pSB4A3;

• Transformation:

-pLac-LacY-GFP on pSB4A3;

• We inoculate:

-pLac-GFP-pTet-LacI (on high copy plasmid);

-pLac-cI-GFP (on low copy plasmid), 3 different inocules using 3 different colonies;

-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).

-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).

10/23/07

10/24/07

10/25/07

10/26/07

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