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Revision as of 15:10, 23 September 2007 (view source) Eismoustique (Talk | contribs) (→Ligations) ← Older edit		Latest revision as of 14:48, 30 September 2007 (view source) Eismoustique (Talk | contribs)
Line 187:		Line 187:
	*L42		*L42
	*L43		*L43
		+
		+
		+	== Sequencing ==
		+
		+	The following sequencing reactions have been performed
		+
		+	TL_MPT5.1+O41
		+	TL_MPT5.2+O41
		+	TL_L26.1+O18
		+	TL_L26.1+O19
		+	TL_L26.2+O18
		+	TL_L26.2+O19
		+	TL_L27.2+O18
		+	TL_L27.2+O19
		+	TL_L27.3+O18
		+	TL_L27.3+O19
		+	TL_L27.4+O18
		+	TL_L27.4+O19
		+	TL_L27.5+O18
		+	TL_L27.5+O19
		+	TL_L28.2+O18
		+	TL_L28.2+O19
		+	TL_L28.3+O18
		+	TL_L28.3+O19
		+	TL_L28.8+O18
		+	TL_L28.8+O19
		+	TL_L29.1+O18
		+	TL_L29.1+O19
		+	TL_L29.2+O18
		+	TL_L29.2+O19
		+	TL_L30A1+O18
		+	TL_L30A1+O19
		+	TL_L30B1+O18
		+	TL_L30B1+O19
		+	TL_L27.2+O24
		+	TL_L27.3+O24
		+	TL_L27.4+O24
		+	TL_L27.5+O24
		+	TL_L28.2+O24
		+	TL_L28.3+O24
		+	TL_L28.8+O24
		+	TL_L28.2+O22
		+	TL_L28.3+O22
		+	TL_L28.8+O22
		+	TL_L28.2+O23
		+	TL_L28.3+O23
		+	TL_L28.8+O23

---

Latest revision as of 14:48, 30 September 2007

yesterday -- tomorrow

Ligations

Ligations
Number	Insert	Insert Volume (µL)	Vector	Vector Volume (µL)	Comments	Number of colonies
C1			MP3.1 digested with EcoRI (D0)	2	control of ligation efficiency
C3			D34 (lox66 BV)	4µl	control of D34 digestion
C5			D50 (BBa_J61002 BV)	1.5µl	control of D50 digestion
C6			D41 (araC/pBad-J61002 plasmid SP dig.)	1µl	control of D41 digestion
C7			D22 (pSB1A2 open vector EP)	4µl	control of D22 digestion
C8			D47 (pSB1A2 XP dig.)	4µl	control of D47 digestion
C9			D48 (pSB1A2 ES dig.)	2µl	control of D48 digestion
L31	D44 (B0015 terminator BI)	4	D35 (lox71 BV)	3	Insertion of B0015 terminator behind lox71	+++
L32	D45 (B0015 terminator FI)	4	D42 (Lox66 FV)	3	Insertion of B0015 terminator in front of lox66	+++
L33	D33.1 (RBS(B0030)CreORF BI)	5	D50 (BBa_J61002 BV)	1.5	pTet>>RBS-Cre	RAS
L34	D49 (lox71-gfptripart BI)	5	D50 (BBa_J61002 BV)	1.5	pTet>>lox71-gfptripart	++
L35	D49 (lox71-gfptripart BI)	5	D41 (araC/pBad-J61002 plasmid SP dig.)	1	araC/pBad>>lox71-gfptripart	+++
L36	D13.4 (mRFP dig. SP)	6	D34 (lox66 BV)	4	lox66-mRFP	+++
L37	D52 (RBS-dapAcoli BI)	6	D34 (lox66 BV)	4	lox66-RBS-dapAcoli	+++
L38	D54 (RBS-dapAsubtilis BI)	13	D34 (lox66 BV)	4	lox66-RBS-dapAsubtilis	RAS
L39	D51 (RBS-dapAcoli BBinsert)	4	D22 (pSB1A2 open vector EP)	4µl	RBS-dapAcoli cloned in pSB1A2	+++
L40	D53 (RBS-dapAsubtilis BBinsert)	13 (	D22 (pSB1A2 open vector EP)	4µl	RBS-dapAsubtilis cloned in pSB1A2	+
L41	D27' (lox71-ftsZ bw-insert)	12	D47 (pSB1A2 XP dig.)	4µl	lox71-ftsZ cloned in pSB1A2	+
L42	D32 (lox71-ftsAftsZ bw-insert)	6	D47 (pSB1A2 XP dig.)	4µl	lox71-ftsA-Z cloned in pSB1A2	+
L43	D55 (RBS-dapAsubtilis FI)	15	D48 (pSB1A2 ES dig.)	2µl	RBS-dapAsubtilis cloned in pSB1A2	+

5 points with :

• L31
• L32
• L34
• L35
• L36
• L37
• L39
• L40 (2 points only)
• L41 (2 points only)
• L42
• L43

Sequencing

The following sequencing reactions have been performed

TL_MPT5.1+O41 TL_MPT5.2+O41 TL_L26.1+O18 TL_L26.1+O19 TL_L26.2+O18 TL_L26.2+O19 TL_L27.2+O18 TL_L27.2+O19 TL_L27.3+O18 TL_L27.3+O19 TL_L27.4+O18 TL_L27.4+O19 TL_L27.5+O18 TL_L27.5+O19 TL_L28.2+O18 TL_L28.2+O19 TL_L28.3+O18 TL_L28.3+O19 TL_L28.8+O18 TL_L28.8+O19 TL_L29.1+O18 TL_L29.1+O19 TL_L29.2+O18 TL_L29.2+O19 TL_L30A1+O18 TL_L30A1+O19 TL_L30B1+O18 TL_L30B1+O19 TL_L27.2+O24 TL_L27.3+O24 TL_L27.4+O24 TL_L27.5+O24 TL_L28.2+O24 TL_L28.3+O24 TL_L28.8+O24 TL_L28.2+O22 TL_L28.3+O22 TL_L28.8+O22 TL_L28.2+O23 TL_L28.3+O23 TL_L28.8+O23