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	1. Mutate global transcription factors		1. Mutate global transcription factors
-	[[Design primers for amplification]]	+	# [[Design primers for amplification]]
-	Perform error-prone PCR	+	# Perform error-prone PCR
	2. Transform mutated genes into E. coli		2. Transform mutated genes into E. coli
-	Choose plasmids	+	# Choose plasmids
-	Restriction enzyme digestion	+	# Restriction enzyme digestion
-	Ligation	+	# Ligation
-	Transformation into E. coli	+	# Transformation into E. coli
	3. Conjugate E. coli with Shewanella		3. Conjugate E. coli with Shewanella

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Revision as of 18:07, 29 May 2007

Home

Welcome to the wiki for Boston University's iGEM 2007 Team!

Our project is aimed at increasing current production from Shewanella Oneidensis by directed evolution of global transcription factors Our plan so far:

1. Mutate global transcription factors

1. Design primers for amplification
2. Perform error-prone PCR

2. Transform mutated genes into E. coli

1. Choose plasmids
2. Restriction enzyme digestion
3. Ligation
4. Transformation into E. coli

3. Conjugate E. coli with Shewanella

4. Screen/select for increased current production due to mutations

Week's Goals

Wednesday 5/30

1. Get all protocols
2. Identify materials/prepare order
3. Design Primers
4. Learn about budget/POs

Thursday 5/31

1. Do primer order
2. Start conjugation practice
3. Confirm restriction enzymes, ligases
4. Order confirmed/needed materials
5. Team Revew Meeting

Friday 6/1

1. Evaluate/continue conjugation, practice electroporation for E. coli
2. Meeting with Tim: Budgets/protocols, Pfizer/fundraising, iGEM registration, beads