BerkiGEM2007Present2
From 2007.igem.org
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[[Image:4maingenes.jpg]][[Image:ThpDarrow.jpg]][[Image:hoxyectoine.jpg]] | [[Image:4maingenes.jpg]][[Image:ThpDarrow.jpg]][[Image:hoxyectoine.jpg]] | ||
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| + | Trehalose is pulled right out of the E.coli genome and consists of the genes otsA and otsB. Naturally otsB comes first because the production of that is more important that otsA so in my operon otsB comes before otsA. | ||
| + | Together the sugar Trehalose is produced which helps the cell recover from freeze-drying. OtsB encodes for a 29.1-kDa trehalose-6-phosphate phosphatase and otsA encodes for a 53.6-kDa trehalose-6-phosphate synthase. Together they make up a Saccharomyces cerevisiae trehalose-6-phosphate synthase/phosphatase complex. Naturally, the 3' end of the otsB coding region overlaps the 5' end of the otsA coding region by 23 nucleotides. The Trehalose helps the frozen dehydrated cells to recover back to a functioning hydrated state. Not allcells survive the freeze dry process but Trehalose increases the successful percentages. Trehalose structure: [[Image:trehalosestructurevp.jpg]] | ||
Revision as of 05:29, 25 September 2007
sorry for the delay. This is a rough draft. we will finish it soon.
Streptomyces Chyrsomallus consists of many genes; however, I was only interested in 4 genes and they are thpA, thpB, thpC and thpD. These 4 genes will help initiate the production of hydroxyectioine. The 4 genes should be arranged in this order: thpA-rbs-thpB-rbs-thpC-rbs-thpD. thpD is the gene that plays a crucial role in the production of hydroxyectoine. The other three genes are there to initiate the process. When inserted into a bacterium, hydroxyectoine will prevent dessication and many other extremes. It will protect the bacteria's proteins and cell membrane.
Trehalose is pulled right out of the E.coli genome and consists of the genes otsA and otsB. Naturally otsB comes first because the production of that is more important that otsA so in my operon otsB comes before otsA. Together the sugar Trehalose is produced which helps the cell recover from freeze-drying. OtsB encodes for a 29.1-kDa trehalose-6-phosphate phosphatase and otsA encodes for a 53.6-kDa trehalose-6-phosphate synthase. Together they make up a Saccharomyces cerevisiae trehalose-6-phosphate synthase/phosphatase complex. Naturally, the 3' end of the otsB coding region overlaps the 5' end of the otsA coding region by 23 nucleotides. The Trehalose helps the frozen dehydrated cells to recover back to a functioning hydrated state. Not allcells survive the freeze dry process but Trehalose increases the successful percentages. Trehalose structure: File:Trehalosestructurevp.jpg
