Glasgow/Drylab full

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|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#99CCFF size=5><b>*Biobricks*<br>Used</b></font>]
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Revision as of 14:12, 27 September 2007

https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg Back To
Glasgow's
Main Page
*Protocols* *References* *Resources* *Orders* *Biobricks*
Used 		*Gels*

Click on a week number for more detailed lab book...


Week 1
Week 2
  • First selection of biobricks transformed.
  • Preparation of stocks.
  • Change of plan because DmpR failed to grow.
  • Researched XylR and DntR sensor systems.
  • Designed primers for DmpR, DntR, XylR and associated promoters.
Week 3
Week 4
  • BioBricks tested by digestion and PCR.
  • PCR with new primers associated with DntR and XylR.
  • *m and *s cloned into TOPO vectors.
  • Primer design for 7 gene operon.
  • DntR cloned into TOPO vector.
  • Primer design for XylR and associated genes.
  • Death gene cloned into TOPO vector.
  • Primer design for death gene.
Week 5
Week 6
  • Suspected amplification of 7 gene operon.
  • *m and *s confirmed as present in TOPO vectors.
  • Confirmed presence of death gene plasmid in some vectors.
  • Miller assay performed with DntR system.
  • Yeast cells.
  • Biobricks made of (*m), (*s), and DntR.
Week 7
Week 8
  • Utilised new biobricks.
  • Site directed mutagenesis of (*m) completed.
  • Site directed mutagenesis of (*s) completed.
  • (*dā†’g*) successfully cloned into TOPO vector.
Week 9
Week 10
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Protocols References Resources Orders Biobricks
Used 		Gels
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