Melbourne/Secondary Reagent LB
From 2007.igem.org
(Difference between revisions)
(→Method:) |
|||
| Line 15: | Line 15: | ||
===Method:=== | ===Method:=== | ||
| - | # | + | #Place Ingredients in a plastic container and add sterile water to the desired volume. |
#Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5. | #Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5. | ||
Revision as of 08:16, 29 September 2007
<return to list of secondary methods> <team home page>
Contents |
Preparation of LB
Uses
- Culturing Bacteria
- Making LB-Agar Plates
Ingredients:
For 1L:
- 10g bacto-tryptone
- 5g yeast extract
- 10g NaCl
- NaOH to adjust pH
Method:
- Place Ingredients in a plastic container and add sterile water to the desired volume.
- Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5.
