Toronto/Lab Notebook
From 2007.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
SUMMARY: | SUMMARY: | ||
| - | + | * Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB | |
| - | + | * NOTE: N1 = J34100 C + S01640 B + B0015 | |
TO DO: | TO DO: | ||
| - | + | * Miniprep all above parts and length check | |
| - | + | * Transform: | |
| - | + | ** J06801 (2005) - it's in the source box, | |
| - | + | ** B0034 - also in | |
== Oct. 1, 2007 == | == Oct. 1, 2007 == | ||
Revision as of 15:25, 3 October 2007
Oct. 2, 2007
Natalie
SUMMARY:
- Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
- NOTE: N1 = J34100 C + S01640 B + B0015
TO DO:
- Miniprep all above parts and length check
- Transform:
- J06801 (2005) - it's in the source box,
- B0034 - also in
Oct. 1, 2007
Time: 3:45 PM - 8:30 PM
Yusuf, Irina
Summary:
- made 5 KAN plates
- made 2 AMP+KAN plates
- made 300 mL LB
Tranformation of following parts:
- I13033 -> KAN
- E0433 -> KAN
- S03140 -> AMP
- J04500 -> KAN
- J31003 -> AMP
- (J23100C + S01640B)+ B0015 -> KAN
Used 4uL of DNA for all transformations
- tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
- couldn't find it in the igem dna box as well !!!
TO DO:
- MP o/n for the above parts
