From 2007.igem.org

Revision as of 15:25, 3 October 2007 (view source) Chuck (Talk | contribs) ← Older edit		Revision as of 15:28, 3 October 2007 (view source) Chuck (Talk | contribs) Newer edit →
Line 17:		Line 17:
	* Transform:		* Transform:
	** J06801 (2005) - it's in the source box,		** J06801 (2005) - it's in the source box,
-	** B0034 - also in	+	** B0034 - also in source box, if not, get it from Registry plates
		+	** Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
		+	* Ligate N1 + S01003
	== Oct. 1, 2007 ==		== Oct. 1, 2007 ==

---

Revision as of 15:28, 3 October 2007

< August | September | Current >

Oct. 2, 2007

Natalie

SUMMARY:

• Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
• NOTE: N1 = J34100 C + S01640 B + B0015

TO DO:

• Miniprep all above parts and length check
• Transform:
  • J06801 (2005) - it's in the source box,
  • B0034 - also in source box, if not, get it from Registry plates
  • Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
• Ligate N1 + S01003

Oct. 1, 2007

Time: 3:45 PM - 8:30 PM

Yusuf, Irina

Summary:

• made 5 KAN plates
• made 2 AMP+KAN plates
• made 300 mL LB

Tranformation of following parts:

• I13033 -> KAN
• E0433 -> KAN
• S03140 -> AMP
• J04500 -> KAN
• J31003 -> AMP
• (J23100C + S01640B)+ B0015 -> KAN

Used 4uL of DNA for all transformations

• tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
• couldn't find it in the igem dna box as well !!!

TO DO:

• MP o/n for the above parts