Week 13
From 2007.igem.org
(Difference between revisions)
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'''Fluorescence test according to [[Bologna University/Fluorescence issue | fluorescence issue protocol]]''' of: | '''Fluorescence test according to [[Bologna University/Fluorescence issue | fluorescence issue protocol]]''' of: | ||
| - | 1. | + | ::1. ''pLac-GFP'': |
| - | -bacteria use to have the PMT output maximum value; | + | ::::-bacteria use to have the PMT output maximum value; |
| - | -normalization of OD value at 0.5; | + | ::::-normalization of OD value at 0.5; |
| - | - output value without IPTG: [0.22, 0.27]V; | + | ::::-output value without IPTG: [0.22, 0.27]V; |
| - | -output value with IPTG: | + | ::::-output value with IPTG: |
| - | + | ::::::::-after 10min: [0.18,0.28]V; | |
| - | + | ::::::::-after 20min: [0.41,0.46]V; | |
| - | + | ::::::::-after 30min: [0.42,0.70]V; | |
| - | + | ::::::::-after 60min: [0.48,0.80]V; | |
| - | -thus we decide to grow the OD value to have higher output value. | + | ::::-thus we decide to grow the OD value to have higher output value. |
| - | 2. pTetR-LacI-pLac-cI-LacY-GFP | + | ::2. pTetR-LacI-pLac-cI-LacY-GFP |
| - | - we observe bacteria vitality, but there isn’t fluorescence after O/N growth with and without progressive induction with IPTG(1mM, 2mM, 3mM, 10mM every 60min). | + | ::::- we observe bacteria vitality, but there isn’t fluorescence after O/N growth with and without progressive induction with IPTG(1mM, 2mM, 3mM, 10mM every 60min). |
| - | - thus we decide to use lactose. | + | ::::- thus we decide to use lactose. |
Revision as of 12:13, 4 October 2007
- 09/24/07
- 09/25/07
- 09/26/07
Fluorescence test according to fluorescence issue protocol of:
- 1. pLac-GFP:
- -bacteria use to have the PMT output maximum value;
- -normalization of OD value at 0.5;
- -output value without IPTG: [0.22, 0.27]V;
- -output value with IPTG:
- -after 10min: [0.18,0.28]V;
- -after 20min: [0.41,0.46]V;
- -after 30min: [0.42,0.70]V;
- -after 60min: [0.48,0.80]V;
- -thus we decide to grow the OD value to have higher output value.
- 1. pLac-GFP:
- 2. pTetR-LacI-pLac-cI-LacY-GFP
- - we observe bacteria vitality, but there isn’t fluorescence after O/N growth with and without progressive induction with IPTG(1mM, 2mM, 3mM, 10mM every 60min).
- - thus we decide to use lactose.
- 2. pTetR-LacI-pLac-cI-LacY-GFP
- 09/27/07
- 09/28/07
