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	=PCR mix=		=PCR mix=
-	5ul 10x buffer	+	*5ul 10x buffer
-	5ul Enhancer	+	*5ul Enhancer
-	0.6ul dNTPs (25mM stock)	+	*0.6ul dNTPs (25mM stock)
-	2.5ul MgSO4 (Supplied in PCR kit)	+	*2.5ul MgSO4 (Supplied in PCR kit)
-	1.5ul Primer I (10uM)	+	*1.5ul Primer I (10uM)
-	1.5ul Primer II (10uM)	+	*1.5ul Primer II (10uM)
-	1ul Template ([[Melbourne/pJS010|pJS010]])	+	*1ul Template ([[Melbourne/pJS010|pJS010]])
-	0.4ul Pfx Platinum (Invitrogen)	+	*0.4ul Pfx Platinum (Invitrogen)
-	32.5ul ddH<math>_{2}</math>	+	*32.5ul ddH<sub>_{2}</sub>

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Revision as of 14:42, 10 October 2007

Protocol for PCR reactions A-G (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

• 5ul 10x buffer
• 5ul Enhancer
• 0.6ul dNTPs (25mM stock)
• 2.5ul MgSO4 (Supplied in PCR kit)
• 1.5ul Primer I (10uM)
• 1.5ul Primer II (10uM)
• 1ul Template (pJS010)
• 0.4ul Pfx Platinum (Invitrogen)
• 32.5ul ddH_{_{2}}