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	====PCR mix====		====PCR mix====
-	*5ul 10x buffer	+	*5ul 10x buffer\\
-	*5ul Enhancer	+	5ul Enhancer
	*0.6ul dNTPs (25mM stock)		*0.6ul dNTPs (25mM stock)
	*2.5ul MgSO4 (Supplied in PCR kit)		*2.5ul MgSO4 (Supplied in PCR kit)

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Revision as of 14:46, 10 October 2007

=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

• 5ul 10x buffer\\

5ul Enhancer

• 0.6ul dNTPs (25mM stock)
• 2.5ul MgSO4 (Supplied in PCR kit)
• 1.5ul Primer I (10uM)
• 1.5ul Primer II (10uM)
• 1ul Template (pJS010)
• 0.4ul Pfx Platinum (Invitrogen)
• 32.5ul ddH₂O

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• 50ul Total