Melbourne/Lab BL Notebook/PCR1
From 2007.igem.org
(Difference between revisions)
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====PCR mix==== | ====PCR mix==== | ||
| - | + | 5ul 10x buffer\\ | |
5ul Enhancer | 5ul Enhancer | ||
| - | + | 0.6ul dNTPs (25mM stock) | |
| - | + | 2.5ul MgSO4 (Supplied in PCR kit) | |
| - | + | 1.5ul Primer I (10uM) | |
*1.5ul Primer II (10uM) | *1.5ul Primer II (10uM) | ||
*1ul Template ([[Melbourne/pJS010|pJS010]]) | *1ul Template ([[Melbourne/pJS010|pJS010]]) | ||
Revision as of 14:46, 10 October 2007
=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
PCR mix
5ul 10x buffer\\ 5ul Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer I (10uM)
- 1.5ul Primer II (10uM)
- 1ul Template (pJS010)
- 0.4ul Pfx Platinum (Invitrogen)
- 32.5ul ddH2O
--
- 50ul Total
