Week 15
From 2007.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
*We inoculate: | *We inoculate: | ||
::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ::-[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid); | ||
| - | ::-[http://partsregistry.org/Part:I763016 I763016](low copy plasmid); | + | ::-[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid); |
::-E.Coli; | ::-E.Coli; | ||
::-[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid). | ::-[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid). | ||
Revision as of 10:46, 12 October 2007
- 10/08/07
- We inoculate:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -E.Coli;
- -[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid).
- Digestion of:
- -[http://partsregistry.org/Part:I763035 I763035] with Eco/Pst1;
- -[http://partsregistry.org/Part:I763007 I763007] with Eco/spe;
- -[http://partsregistry.org/Part:I763026 I763026] with Eco/Xba;
- -[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Spe/Xba.
- Ligation for:
- -[http://partsregistry.org/Part:I763035 I763035]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:I763026 I763026];
- -[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
- Run on electrophoresis gel.
- [http://partsregistry.org/Part:I763007 I763007] and [http://partsregistry.org/Part:I763026 I763026] band extraction.
- O/N ligation for:
- -[http://partsregistry.org/Part:I763026 I763026]+[http://partsregistry.org/Part:I763007 I763007];
- -[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763007 I763007]+[http://partsregistry.org/Part:pSB4A3 pSB4A3].
- We inoculate:
- -[http://partsregistry.org/Part:I763031 I763031](low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016](low copy plasmid);
- -[http://partsregistry.org/Part:I763032 I763032] (high copy plasmid).
- 10/09/07
- Transformation of:
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007];
- -[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid);
- -[http://partsregistry.org/Part:I763032 I763032] (low copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid).
- We inoculate in 6ml:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid).
- Ligation for:
- -[http://partsregistry.org/Part:I763016 I763016] (Eco/Spe)+GFP.(Eco/Xba).
- 10/10/07
- Miniprep of:
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid).
- Digestion for :
- -[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid) with Spe/Pst1;
- Run on electrophoresis gel and [http://partsregistry.org/Part:I763016 I763016] band extraction.
- Ligation for :
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid)+GFP;
- -[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:C0012 C0012] (high copy plasmid).
- We inoculate in 6ml:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
- -[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid).
- 10/11/07
- Stock preparation (500ul) of:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007](high copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
- -[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid).
- Miniprep of:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763016 I763016] (low copy plasmid);
- -[http://partsregistry.org/Part:I763026 I763026]+ [http://partsregistry.org/Part:I763007 I763007](high copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid);
- -[http://partsregistry.org/Part:I763024 I763024] (low copy plasmid).
- Fluorescence test of:
- -[http://partsregistry.org/Part:I763031 I763031] (low copy plasmid);
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007] (high copy plasmid);
- -[http://partsregistry.org/Part:I763007 I763007] (low copy plasmid).
- Control digestion of:
- -[http://partsregistry.org/Part:I763031 I763031] with Xba/Pst1;
- -[http://partsregistry.org/Part:I763016 I763016] with Xba/Pst1 and Spe/Xba;
- -[http://partsregistry.org/Part:I763026 I763026] + [http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1 and Spe/Xba;
- -[http://partsregistry.org/Part:I763024 I763024] with Xba/Pst1;
- -[http://partsregistry.org/Part:I763007 I763007] with Xba/Pst1.
- Run on electrophoresis gel and band extraction.
- Transformation of:
- -[http://partsregistry.org/Part:I763019 I763019];
- -[http://partsregistry.org/Part:I763024 I763024]+[http://partsregistry.org/Part:C0012 C0012];
- -[http://partsregistry.org/Part:I763032 I763032].
- We inoculate
- -[http://partsregistry.org/Part:I763019 I763019].
- 10/12/07
- Miniprep of:
- -[http://partsregistry.org/Part:I763019 I763019].
- Digestion of:
- -[http://partsregistry.org/Part:I763019 I763019] with Xba/Pst1;
- -[http://partsregistry.org/Part:I763019 I763019] with Eco/Xba.
- Run on electrophoresis gel.
