PHO80cds

From 2007.igem.org

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'''Gene Pho80
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GenePho
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CODING SEQUENCE'''
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CODINGSEQUENCE
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ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATGAGGATCAAGG
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ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATGAGGATCAAGGGATACCAAAAGTAATTCTGCCCGCTGATTTTAATAAATGCTCTAGAACTGACCTAGTGGTGCTCATATCACGAATGTTAGTATCGCTGATAGCAATCAATGAAAATTCAGCAACAAAGAAATCTGATGACCAAATTACTTTAACACGATACCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTATTTCATACGACTGACAAAGTTTTCCTCTTTAGAACATTGTGTGCTTATGACATCACTCTATTATATCGATTTATTGCAAACTGTGTATCCTGATTTTACGCTTAATTCGTTGACTGCCCATAGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCTTATGTGATTCGTTCTCAACAAACGCCCATTATGCAAAAGTTGGAGGAGTACGATGTCACGAATTGAATATACTGGAGAACGATTTTTTAAAGAGAGTAAACTACAGAATCATTCCGCGGGATCATAACATTACGTTATGTAGTATAGAGCAAAAACAGAAAAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGATTTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGATATAATGTTCTAGATAAATACTATCGAAGAATAGTTCAGCTGGTGGGTTCCTTTAACGCTTCACCTGATAAGAGTAGAAAGGTTGATTATGTTCTCCCGCCAAATATTGATATAGTGAGTGAAAGTGGCTCCCAAACTACTCAACTAAAGGGGTCGTCATCACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCAAAGGACGCACATATCTATAACAAGCGATCAAAGCCAGATTAA
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GATACCAAAAGTAATTCTGCCCGCTGATTTTAATAAATGCTCTAGAACTG
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ACCTAGTGGTGCTCATATCACGAATGTTAGTATCGCTGATAGCAATCAAT
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GAAAATTCAGCAACAAAGAAATCTGATGACCAAATTACTTTAACACGATA
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CCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTATTTCATACGAC
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TGACAAAGTTTTCCTCTTTAGAACATTGTGTGCTTATGACATCACTCTAT
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TATATCGATTTATTGCAAACTGTGTATCCTGATTTTACGCTTAATTCGTT
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GACTGCCCATAGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCT
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TATGTGATTCGTTCTCAACAAACGCCCATTATGCAAAAGTTGGAGGAGTA
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CGATGTCACGAATTGAATATACTGGAGAACGATTTTTTAAAGAGAGTAAA
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CTACAGAATCATTCCGCGGGATCATAACATTACGTTATGTAGTATAGAGC
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AAAAACAGAAAAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGAT
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TTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGATATAATGTTCT
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AGATAAATACTATCGAAGAATAGTTCAGCTGGTGGGTTCCTTTAACGCTT
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CACCTGATAAGAGTAGAAAGGTTGATTATGTTCTCCCGCCAAATATTGAT
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ATAGTGAGTGAAAGTGGCTCCCAAACTACTCAACTAAAGGGGTCGTCATC
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ACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCAAAGGACGCAC
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ATATCTATAACAAGCGATCAAAGCCAGATTAA
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XbaI cuts in 2 sites 91 and 648
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XbaI restriction sequence TCTAGA
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cuts in sites
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91
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648
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'''Pho80cds'''
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Pho cds
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882 bp e 293aa
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882bp e 293aa
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XbaI restriction sequence TCTAGA
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1)91 bp position 31-32aa SR
1)91 bp position 31-32aa SR
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S=TCT  TCC  TCA  TCG
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S=TCTTCCTCATCG
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R=AGA  AGG
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R=AGAAGG
2)648 bp position 216-217-218aa VLD
2)648 bp position 216-217-218aa VLD
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V=TT    GTC    GTA    GTG
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V=TTGTCGTAGTG
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L=TTA  TTG    CTT    CTA  CTC  CTG
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L=TTATTGCTTCTACTCCTG
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D=GAT  GAC
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D=GATGAC
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5'3' Frame 1
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Frame 5’-3’
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MetESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMetLVSLIAINENSATKKSDDQITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMetTSLYYIDLLQTVYPDFTLNSLTAHRFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLCSIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRKVDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPDStop
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Met E S T S G E R S E N I H E D Q G I P K V I L P A D F N K C S R T D L V V L I S R Met L V S L I A I N E N S A T K K S D D Q I T L T R Y H S K I P P N I S I F N Y F I R L T K F S S L E H C V L Met T S L Y Y I D L L Q T V Y P D F T L N S L T A H R F L L T A T T V A T K G L C D S F S T N A H Y A K V G G V R C H E L N I L E N D F L K R V N Y R I I P R D H N I T L C S I E Q K Q K K F V I D K N A L G S L D L D S Y S Y V N R P K S G Y N V L D K Y Y R R I V Q L V G S F N A S P D K S R K V D Y V L P P N I D I V S E S G S Q T T Q L K G S S S P N S H S S Q K R Y S E A K D A H I Y N K R S K P D Stop
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VirtualSequence:
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SEQUENCEAA;MW;CAFAECCRC.
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MESTSGERSENIHEDQGIPKVILPADFNKC[[SR]]TDLVVLISRMLVSLIAINENSATKKSDD
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QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH
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RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC
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SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYN[[VLD]]KYYRRIVQLVGSFNASPDKSRK
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VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD
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Virtual Sequence:
 
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SEQUENCE  293 AA;  33227 MW; 72918CAF845AE74C CRC64.
 
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    MESTSGERSE NIHEDQGIPK VILPADFNKC [[SR]]TDLVVLIS RMLVSLIAIN ENSATKKSDD
 
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    QITLTRYHSK IPPNISIFNY FIRLTKFSSL EHCVLMTSLY YIDLLQTVYP DFTLNSLTAH
 
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    RFLLTATTVA TKGLCDSFST NAHYAKVGGV RCHELNILEN DFLKRVNYRI IPRDHNITLC
 
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    SIEQKQKKFV IDKNALGSLD LDSYSYVNRP KSGYN[[VLD]]KY YRRIVQLVGS FNASPDKSRK
 
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    VDYVLPPNID IVSESGSQTT QLKGSSSPNS HSSQKRYSEA KDAHIYNKRS KPD
 

Revision as of 11:15, 17 October 2007

GenePho CODINGSEQUENCE

ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATGAGGATCAAGGGATACCAAAAGTAATTCTGCCCGCTGATTTTAATAAATGCTCTAGAACTGACCTAGTGGTGCTCATATCACGAATGTTAGTATCGCTGATAGCAATCAATGAAAATTCAGCAACAAAGAAATCTGATGACCAAATTACTTTAACACGATACCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTATTTCATACGACTGACAAAGTTTTCCTCTTTAGAACATTGTGTGCTTATGACATCACTCTATTATATCGATTTATTGCAAACTGTGTATCCTGATTTTACGCTTAATTCGTTGACTGCCCATAGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCTTATGTGATTCGTTCTCAACAAACGCCCATTATGCAAAAGTTGGAGGAGTACGATGTCACGAATTGAATATACTGGAGAACGATTTTTTAAAGAGAGTAAACTACAGAATCATTCCGCGGGATCATAACATTACGTTATGTAGTATAGAGCAAAAACAGAAAAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGATTTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGATATAATGTTCTAGATAAATACTATCGAAGAATAGTTCAGCTGGTGGGTTCCTTTAACGCTTCACCTGATAAGAGTAGAAAGGTTGATTATGTTCTCCCGCCAAATATTGATATAGTGAGTGAAAGTGGCTCCCAAACTACTCAACTAAAGGGGTCGTCATCACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCAAAGGACGCACATATCTATAACAAGCGATCAAAGCCAGATTAA

XbaI restriction sequence TCTAGA cuts in sites  91  648


Pho cds 882bp e 293aa

1)91 bp position 31-32aa SR S=TCTTCCTCATCG

R=AGAAGG

2)648 bp position 216-217-218aa VLD V=TTGTCGTAGTG

L=TTATTGCTTCTACTCCTG

D=GATGAC


Frame 5’-3’ MetESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMetLVSLIAINENSATKKSDDQITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMetTSLYYIDLLQTVYPDFTLNSLTAHRFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLCSIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRKVDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPDStop

VirtualSequence: SEQUENCEAA;MW;CAFAECCRC. MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLVSLIAINENSATKKSDD QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRK VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD


PHO80 Primer sequences:

t93c 5'-cgctgattttaataaatgctccagaactgacctagtggtgc-3'

t93c antisense 5'-gcaccactaggtcagttctggagcatttattaaaatcagcg-3'

t648c 5'-atcgtccaaaaagtggatataatgtcctagataaatactatcgaagaatag-3'

t648c antisense 5'-ctattcttcgatagtatttatctaggacattatatccactttttggacgat-3'

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