NYMU Taipei/Our Part Registry

1

2

3

4

5

6

7

8

9

10

 A

pOmpC (P.E.)

Tar-EnvZ (P.E.)

EYFP, J04086 (P.E.)

EYFP, E0032 (P.E.)

RBS (P.E.)

HSL (P.E.)

CinR (P.E.)

pCinRHSL (P.E.)

D-term (P.E.)

pCinRHSL RBS+ (P.E.)

 B

pOmpC (P.E. #1)

pOmpC (P.E. #2)

 pOmpC (P.E. #3)

pOmpC (P.E. #4)

 H_INS (P.E.)

M_IDE (P.E.)

H_IGFBP3 (P.E.)

 H_IGFBP7 (P.E.)

RBS (P.E.)

CinR (P.E.) 

 C

HSL (P.E.) 

D-term (P.E.)

ECFP RBS+ (P.E.)

pCinRHSL (P.E.) 

EYFP RBS+ (P.E.) 

 pLacIQ (P.E.)

Tar-EnvZ (P.E.)

pCinRHSL RBS+ (P.E.) 

 CinR+HSL (P.E. #1)

 D

 CinR+HSL (P.E. #3)

CinR+HSL (P.E. #4)
empty

 RBS+CinR (P.E. #1)

 RBS+CinR (P.E. #2)

 RBS+CinR (P.E. #3)

 RBS+CinR (P.E. #4)

 H_INS_A (D. XP #1)

H_INS_A (D. XP #2) 

 E

 pOmpC (D. SP)

RBS (D. SP)

CinR (D. XP)

HSL (D. XP)

EYFP RBS+ (D. XP)

H_INS_B (D. XP #1)

H_INS_B (D. XP #2)

H_INS_B (D. XP #3)

H_INS_B (D. XP #4)

H_INS_B (D. XP #5)

 F

 H_INS_A (PCR #1)

H_INS_A (PCR #3) 

H_INS_B (PCR #1)

 H_INS_B (PCR #2)

H_INS_B (PCR #3)

 H_INS_B (PCR #4)

 H_INS_B (PCR #5)

 G

pOmpC+EYFP (P.E. #1-1) 

pOmpC+EYFP (P.E. #1-2)

 pOmpC+EYFP (P.E. #2)

 pOmpC+EYFP (P.E. #3)

 pOmpC+EYFP (P.E. #4)

 pOmpC+EYFP (P.E. #5)

pOmpC+EYFP (P.E. #6)

 RBS+HSL (P.E. #1)

RBS+HSL (P.E. #2)

RBS+HSL (P.E. #3)

 H

 RBS+HSL (P.E. #4)

 CinR+HSL (ligation)

RBS+CinR (P.E. #1)

RBS+CinR (P.E. #2)

RBS+CinR ver.2 (P.E.)

pOmpC+H_INS_A (ligation,9/8)

pOmpC+H_INS_B (ligation,9/8)

 D-term (D. EX)

CinR+HSL (D. ES #3) OD = 0

CinR+HSL (D.  ES #4) C = 0.02 ug/uL

 I

 RBS+CinR (D. SP)

RBS+HSL (D. XP) 

 CinR+HSL+D-term (P.E. #1)

 CinR+HSL+D-term (P.E. #3)

 CinR+HSL+D-term (P.E. #4)

 pOmpC+H_INS_A (ligation, 9/9)

 TATD_IDE (PCR)

D-term (D. EX) 

 CinR+HSL + D-term (ligation)

CinR+HSL (D. E) 

 J

 EYFP RBS+ (P.E.)

 pTet GFP (<a href="http://partsregistry.org/Part:BBa_I13522">I13522</a>, 13C)

p(Las)TetO (<a href="http://partsregistry.org/Part:BBa_I14015">I14015</a>) 

 pTet GFP (<a href="http://partsregistry.org/Part:BBa_I13522">I13522</a>, 6N)

CFP + YFP (<a href="http://partsregistry.org/Part:BBa_I13605">I13605</a>, 8B) 

pTet GFP (<a href="http://partsregistry.org/Part:BBa_I13522">I13522</a>, 15H)

pLacIQ (P.E.)

Riboswitch (<a href="http://partsregistry.org/Part:BBa_J04686">J04686</a>, 12M)

 tetR (<a href="http://partsregistry.org/Part:BBa_C0040">C0040</a>, 5C)

 ECFP RBS+ (P.E.)

1

2

3

4

5

6

7

8

9

10

 A

pCinRHSL (P.E. #1) 

pCinRHSL (P.E. #1)

pOmpC (P.E. #1) 

 pOmpC (P.E. #2)

pOmpC (P.E. #3)

 pOmpC (P.E. #4)

pCinRHSL + OmpRBS (gel)

 B

pCinRHSL (D. SP) 

 OmpRBS (D. XP)

 pOmpC (D. SP)

 pCinRHSL + OmpRBS (plasmid clone #7)

 pCinRHSL + OmpRBS (plasmid clone #10)

 C

 D

 E

 F

 G

 H

 I

 INSA_F PCR primer

 INSA_R PCR primer

 OmpRBS_F primer

OmpRBS_R primer

 TAT_F PCR primer

 TATA_R PCR primer

TATB_R PCR primer

TATD_R PCR primer

 INSB_F PCR primer

 INSB_R PCR primer

 IDE_F PCR primer

IDE_R PCR primer