Arthur Yu Notebook
From 2007.igem.org
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* programmed pcr on machine upstairs (#6) | * programmed pcr on machine upstairs (#6) | ||
* we got computers | * we got computers | ||
| - | * AGAR SUX | + | * AGAR SUX, for future reference: |
| + | # nuke @ 20:00 min, 50% power. | ||
| + | # water bath in tap water for 5-10 min | ||
| + | # thaw the antibiotic right now!! | ||
| + | # FIRE for disinfecting | ||
| + | # pour that stuff. set 15 min, then marker it then bag | ||
| + | |||
==6/6 waiting for oligos== | ==6/6 waiting for oligos== | ||
* Made oligos and constructs with Vai, for getting wbbL and neuS from pJ23006-Bca9106 | * Made oligos and constructs with Vai, for getting wbbL and neuS from pJ23006-Bca9106 | ||
Revision as of 01:57, 8 June 2007
My Construction Files
My Sequencing Files
6/7 we got benches
- we got benches
- pcr of [http://partsregistry.org/Part:BBa_I716253:Design HPI/katG from Salmonella]
- well... getting the mutated PCR prod overnight. going to xform tmrw, hope it works!
- programmed pcr on machine upstairs (#6)
- we got computers
- AGAR SUX, for future reference:
- nuke @ 20:00 min, 50% power.
- water bath in tap water for 5-10 min
- thaw the antibiotic right now!!
- FIRE for disinfecting
- pour that stuff. set 15 min, then marker it then bag
6/6 waiting for oligos
- Made oligos and constructs with Vai, for getting wbbL and neuS from pJ23006-Bca9106
- We tried the P_tet/RFP triple/double digest to make a composite part.
- FAIL
- probably source DNA is bad
- so much for that activity...
Other stuff: I won speed scrabble. even though I kind of cheated ish (didn't stop when Sam said stop"
6/5 coolbeans
- Finalized oligos to order with Vai
- Learned about LB broth-ing and LB/Agar plating. Thanks, Austin and Sam :)
- Learned about the many composite part-making methods. Props 2 Chris
- prefix/suffix is weaksauce
- Use the AlwnI or BsaI or BglI, in conjuction with BglII or BamHI << (Did this today)
- DBBS
- 3 antibiotic; MIT endorses, used for BioBrick 1.0. Triple digest = bad
- 1-2-3 method << 'Our Goal' in a few weeks. should be leet.
- Planned and vicariously did the making of P_tet+RFP brick (see Vai Notebook)
Other Notes: All oligos are being ordered, w00t w00t.
Ayu 18:36, 5 June 2007 (EDT)
6/4 Training Finishes, Real Stuff Starts
- Incubated some colonies
- Miniprep'd already-been-incubated colonies (2)
- Double digest of the 2 minipreps + parent plasmid
- Colony PCR'd the incubated E.coli
- Ran gel of the digest + PCR
- >>> PCR product / Miniprep 1 / Parent Plasmid / Miniprep 2 / Ladder >>>
- No bands for PCR or parent. Confused? Other ones look great.
As for me: Wiki acc works now.
Designing oligos and will compare with Vai.
Ayu 18:19, 4 June 2007 (EDT)
to do
