USTC/SuXiaofeng
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'''(C)'''I've develop the selection work by the means of Blue White Selection on top agar Luria-Bertani broth to obtain the R-P pairs.Below are some pictures from my experiment design and experiment work. | '''(C)'''I've develop the selection work by the means of Blue White Selection on top agar Luria-Bertani broth to obtain the R-P pairs.Below are some pictures from my experiment design and experiment work. | ||
| - | [[Image:USTC_allen6]][[Image:USTC_allen7]] | + | [[Image:USTC_allen6.jpg]] [[Image:USTC_allen7.jpg]] |
| - | [[Image:USTC_allen8]][[Image:USTC_allen9]] | + | [[Image:USTC_allen8.jpg]] [[Image:USTC_allen9.jpg]] |
*;Key Results | *;Key Results | ||
Revision as of 17:01, 20 October 2007
XIAOFENG SU
Undergruduate Student of Cellular and Molecular Biology,USTC
Email: allensue@mail.ustc.edu.cn (preference) or xiaofsu@gmail.com
Phone: +86-551-3602469 (Lab)
Mobile: +86-13866722084
Address: Room 439, School of Life Sciences, USTC, Hefei, Anhui, P.R.China, 230026
Research Interest
- Directed Evolution for Seeking New Protein-DNA Interactions
- Approaches of Synthetic Biology for Forming Novel "Genetic Engineering Machines"
- Differentiation and Development of Stem Cells
Research Work
- Overall Description
For obtaining the signaling transduction parts of repression with high fidelity, I've experimentally designed and acquired some specific repressor-promoter pairs(R-P pairs or P-R pairs)based on Lactose Operon by directed evolution on plate. Besides, through quantitative assay,the novel artificial R-P pairs I selected have been tested for their binding performance so that P-R pairs of highest affinity and specificity can make a figure out of P-R pair candidates. Most of the parts in my work have been BioBricks-Standardized and work as BioBrick parts.
- Experimental Design
- Construction of Expression Library of Lac-Repressor Family [Collaboration]
- Synthesis of Promoter Sequence with Specific Operators
- Construction of Low-copy Reporter System with Specific Opertors
- Selection of Promoter-Repressor Pair(P-R Pair) including re-testing validity of these combination
- Transfering the Operators to Double Reporter Systems [Collaboration]
- Quantitative assay of Repression Intensity and Specificity of P-R Pairs
- Results From RM(Repression Matrix) to ORM(Orthogonal RM)
- Work Process and Summary
(B)Within the work of establishment of two selection systems, a time consuming step is synthesis of target specific promoters that contain the mutated site design by Do. On account of economic and convenient aspect, I use 2-Step unparallel PCR by 3 fragments of primers and 2 times of PCR with different reaction conditions.As Below.
(C)I've develop the selection work by the means of Blue White Selection on top agar Luria-Bertani broth to obtain the R-P pairs.Below are some pictures from my experiment design and experiment work.
- Key Results
