USTC/Demonstration
From 2007.igem.org
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* can be "resetted" by IPTG signal (i.e. all outputs lighten when IPTG added in); | * can be "resetted" by IPTG signal (i.e. all outputs lighten when IPTG added in); | ||
* is expected to output the results as the truth table shown in Figure 2. | * is expected to output the results as the truth table shown in Figure 2. | ||
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| + | =Logic Abstract of the Demo= | ||
[[Image:DemonstrationLogic.png|thumb|512px|left|'''Figure 1''' The logic diagram of the demo system.]] | [[Image:DemonstrationLogic.png|thumb|512px|left|'''Figure 1''' The logic diagram of the demo system.]] | ||
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<br style="clear:both;"> | <br style="clear:both;"> | ||
| - | [[Image:DemonstrationTruthTable.png|thumb| | + | [[Image:DemonstrationTruthTable.png|thumb|320px|left|'''Figure 2''' The truth table of the demo system]] |
| - | + | ||
<br style="clear:both;"> | <br style="clear:both;"> | ||
| - | + | In this truth table we can see that IPTG might result in all outputs lighten whether aTc or AHL exsit. (It just like the "8888..." on the screen when you reset your calculator or some other digital equipments.) | |
| + | |||
| + | =Actual System= | ||
| + | [[Image:DemonstrationSystem.png|thumb|512px|left|'''Figure 3''' The signal pathway of the demo system.]] | ||
<br style="clear:both;"> | <br style="clear:both;"> | ||
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| + | The left three parts are loaded on [http://partsregistry.org/Part:BBa_I732998 pSB1A3-I732998], | ||
| + | and the rest two parts are loaded on [http://partsregistry.org/Part:BBa_I732999 pSB1A3-I732999]. We've built up this system in one kind of TOP10 E.coli, but it seems that it cannot output FP stably when aTc exsit | ||
Revision as of 19:40, 21 October 2007
An actual demonstration is decided to show the extensible ability of our method.
This demo system
- is designed as simple as possible, without no "cool" logic function;
- includes all the three logic gates, which form into a three-level logic circuit;
- shows that wires can cross and branch off (without interference);
- is loaded on two plasmids, [http://partsregistry.org/Part:BBa_I732998 pSB1A3-I732998] and [http://partsregistry.org/Part:BBa_I732999 pSB1A3-I732999] ,and transformed into Top10 strain in practice;
- accepts aTc and AHL signals as inputs;
- produces RFP and GFP signals as outputs;
- can be "resetted" by IPTG signal (i.e. all outputs lighten when IPTG added in);
- is expected to output the results as the truth table shown in Figure 2.
Logic Abstract of the Demo
In this truth table we can see that IPTG might result in all outputs lighten whether aTc or AHL exsit. (It just like the "8888..." on the screen when you reset your calculator or some other digital equipments.)
Actual System
The left three parts are loaded on [http://partsregistry.org/Part:BBa_I732998 pSB1A3-I732998], and the rest two parts are loaded on [http://partsregistry.org/Part:BBa_I732999 pSB1A3-I732999]. We've built up this system in one kind of TOP10 E.coli, but it seems that it cannot output FP stably when aTc exsit
