Tokyo/New AHL check
From 2007.igem.org
(Difference between revisions)
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===Purpose: === | ===Purpose: === | ||
| - | + | To determine and compare the activity of the AHL used so far and that of the newly perchased AHL. | |
===Samples: === | ===Samples: === | ||
| - | + | A4 placQI in pTrc99A (pos. con.) | |
<br>A4ΔP in pTrc99A (neg. con.) | <br>A4ΔP in pTrc99A (neg. con.) | ||
<br>A4 hybrid promoter in pBR322 | <br>A4 hybrid promoter in pBR322 | ||
===Procedure: === | ===Procedure: === | ||
| - | + | The old and the newly delivered AHL was used. | |
<br>[[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]] | <br>[[Tokyo/AHL assay Standard protocol |AHL assay Standard protocol ]] | ||
<br>The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM. | <br>The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM. | ||
===Result & Conclusion: === | ===Result & Conclusion: === | ||
Revision as of 11:22, 24 October 2007
Contents |
Purpose:
To determine and compare the activity of the AHL used so far and that of the newly perchased AHL.
Samples:
A4 placQI in pTrc99A (pos. con.)
A4ΔP in pTrc99A (neg. con.)
A4 hybrid promoter in pBR322
Procedure:
The old and the newly delivered AHL was used.
AHL assay Standard protocol
The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM.
