Chiba/Sandbox/Home2

From 2007.igem.org

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[[Chiba/Introduction|Introduction]] || [[Chiba/Project_Design|Project Design]] | [[Chiba/Engeneering_Flagella|Engeneering Flagella]] | [[Chiba/Quorum_Sensing|Quorum Sensing]] || [[Chiba/Goal|Our Goal]] || [[Chiba/Team_Members|Team Members]]
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[[Chiba|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1.Affinity Tag]] | [[Chiba/Communication|2.Communication Module]] | [[Chiba/Quorum_Sensing|3.Size Control]] ) | [[Chiba/Making Marimo|Making Marimos]] | [[Chiba/Goal|Our Goal]]<br>
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[[Chiba/Acknowledgements|Acknowledgements]] | [[Chiba/Team_Members|Team Members]] | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | [[Chiba/Members_Only|メンバ連絡簿]]  
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==Concept==
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==Chiba's Project==
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[[Image:chiba_marimobig.jpg|frame|Marimo in the lake]]
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Our iGEM project is to make a '''Marimo-ish gathering of bacteria'''. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?)
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Chiba University iGEM07 team's project is to make a '''''Marimo-ish gathering of bacteria'''''. ''Marimo'' is a green spherical shaped algae, which is a popular living organism in Japan as a National Treasure. When you come to Japan, you absolutely come across to Marimo in Aquarisms, TV programs or Mangas :)
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Motivation.<br>
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(more...)
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==Design==
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Our marimo bacteria requres as follows:
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===How Marimos are Made===
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{| border="1"
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球形の毬藻は，他の崩れた毬藻断片を核として放射状に伸びることによって出来る．よって成長後の球形毬藻は全て一様で核が存在しない．ちなみに最初の無核球形毬藻は，他の泥や砂などを核として成長し崩れた毬藻断片を核としたと考えられている．（イメージ図）
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Affinity Tag
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Communication Module
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Size Controler
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[[Chiba/Marimo|more about marimos...]]
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これを組み合わせて以下のように毬藻をつくる：
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References:
 
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*Yoshio Sakai, Marimo no Kagaku, 北海道大学図書刊行会, 1991
 
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==Achievements Overview==
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===Making Affinity Tags===
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#Tagとなるヒスチジンペプチドが入った鞭毛をつくる．
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#*発現をチェック
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#*ディスプレイをチェック
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===Making Communication Module===
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#Making Senders
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#*1.---
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#*2.---
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#Making Receivers
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#*1.---
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#*2.---
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#*
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#*
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===Controlling Size===
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#Improve Sender
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#*前駆体合成酵素metKを入れてみた．＞結果？
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#Improve Receiver
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#*LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
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#Localize AHL
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#*aiiAその１の回路（〜〜になるはず）＞結果
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#*aiiAその２の回路
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#*その３
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===Making Marimo===
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===Why Make a "Marimo Bacteria"?===
 
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大腸菌は固体上やプレート上でそれぞれバイオフィルムやコロニーを形成するなど，固体上で集合体を生じることは知られている．しかし液体中で細胞自身が集合する例は調べる限りでは見当たらない．我々は大腸菌のみで毬藻のような独立した有限の形の集合体をつくることを目指した．
 
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==Project==
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==Remaining Task(Work) ==
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===What our system requires===
 
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{| style="border:0px;"
 
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1.Adhesives of Bacteria: make a '''His-tagged Flagella'''
 
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[[Image:Chiba_stickbacteria.png]]
 
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| width="50%" valign="top" |
 
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2.Size Controller: Generate '''AHL Concentration''' in Quorum Sensing
 
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（絵）
 
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|}
 
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===Genetic Circuit===
 
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[[Image:Chiba_marimosystem.png]]
 
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====Sender====
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==Our Goal==
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====Reciever====
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==Future==

Revision as of 03:18, 27 October 2007

Chiba logo.png

Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal
Acknowledgements | Team Members | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | メンバ連絡簿

Chiba's Project

Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?) Motivation.
(more...)

Design

Our marimo bacteria requres as follows:

Affinity Tag

Communication Module

Size Controler

これを組み合わせて以下のように毬藻をつくる：


Achievements Overview

Making Affinity Tags

  1. Tagとなるヒスチジンペプチドが入った鞭毛をつくる．
    • 発現をチェック
    • ディスプレイをチェック

Making Communication Module

  1. Making Senders
    • 1.---
    • 2.---
  2. Making Receivers
    • 1.---
    • 2.---

Controlling Size

  1. Improve Sender
    • 前駆体合成酵素metKを入れてみた．＞結果？
  2. Improve Receiver
    • LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
  3. Localize AHL
    • aiiAその１の回路（〜〜になるはず）＞結果
    • aiiAその２の回路
    • その３

Making Marimo

Remaining Task(Work)

Our Goal

Future

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