Tokyo/Assay
From 2007.igem.org
(Difference between revisions)
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導出に関してはfor more detail. | 導出に関してはfor more detail. | ||
- | = [[Tokyo/Assays for hybrid promoter|Assays for hybrid promoter]] = | + | == [[Tokyo/Assays for hybrid promoter|Assays for hybrid promoter]] == |
These assays were done | These assays were done | ||
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== [[Tokyo/Repression and activation check with LacI and AHL|Repression and activation check with LacI and AHL]] == | == [[Tokyo/Repression and activation check with LacI and AHL|Repression and activation check with LacI and AHL]] == | ||
+ | 目的 | ||
+ | 結果 | ||
== [[Tokyo/IPTG assay|IPTG assay]] == | == [[Tokyo/IPTG assay|IPTG assay]] == | ||
+ | 目的 | ||
+ | 結果 | ||
== [[Tokyo/Activation check by cell-produced AHL |Activation check by cell-produced AHL ]] == | == [[Tokyo/Activation check by cell-produced AHL |Activation check by cell-produced AHL ]] == | ||
+ | 目的 | ||
- | + | 結果 | |
- | + | ||
=2.Preliminary assays= | =2.Preliminary assays= | ||
====Preliminary assays is [[Tokyo/Preliminary assays |here]]!!==== | ====Preliminary assays is [[Tokyo/Preliminary assays |here]]!!==== |
Revision as of 11:16, 22 October 2007
1.Important assay
Hill function fitting
新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める.
ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
導出に関してはfor more detail.
Assays for hybrid promoter
These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.
Repression and activation check with LacI and AHL
目的
結果
IPTG assay
目的
結果
Activation check by cell-produced AHL
目的
結果