USTC/OperatorPosition
From 2007.igem.org
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+ | Operator positioning plays a role in determining in vivo level of repression.[[USTC/OperatorPosition#References|[1]]]Both operators with strong and weak repression are required to the gates . So we tested the positioning effects systematically. | ||
[[Image:USTC_RelativePromoterActivity.png|thumb|600px|center|'''Figure 1''' Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence. | [[Image:USTC_RelativePromoterActivity.png|thumb|600px|center|'''Figure 1''' Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence. | ||
Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence. | Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence. | ||
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[[Image:USTC_EffectOfPosition.png|thumb|600px|center|'''Figure 2''' Assumed influence on solo-repression when an operator is at a different position.]] | [[Image:USTC_EffectOfPosition.png|thumb|600px|center|'''Figure 2''' Assumed influence on solo-repression when an operator is at a different position.]] | ||
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+ | == References == | ||
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+ | 1. Elledge, S. J. & Davis, R. W. (1989), 'Position and density effects on repression by stationary and mobile DNA-binding proteins.', <i>Genes Dev</i> 3(2), 185--197. |
Revision as of 06:08, 26 October 2007
Operator positioning plays a role in determining in vivo level of repression.[1]Both operators with strong and weak repression are required to the gates . So we tested the positioning effects systematically.
References
1. Elledge, S. J. & Davis, R. W. (1989), 'Position and density effects on repression by stationary and mobile DNA-binding proteins.', Genes Dev 3(2), 185--197.