Freiburg07/Transformation
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1. Thaw frozen cells.<BR> | 1. Thaw frozen cells.<BR> | ||
| - | 2. Add 10 µl ligation and shake | + | 2. Add 10 µl ligation and shake gently.<BR> |
3. Incubate on ice for 5-10 minutes.<br> | 3. Incubate on ice for 5-10 minutes.<br> | ||
Revision as of 14:54, 24 October 2007
Transformation of frozen competent cells
1. Thaw frozen cells.
2. Add 10 µl ligation and shake gently.
3. Incubate on ice for 5-10 minutes.
4. Heat shock cells for 10 minutes at 37 °C.
5. Bring up in 1 mL LB and shake gently for 1 hour at 37 °C.
