ETHZ/primer pcr killspe up
From 2007.igem.org
< ETHZ(Difference between revisions)
| Line 2: | Line 2: | ||
The sequence for the Primer to "kill" the SpeI site in the origin of replication is: | The sequence for the Primer to "kill" the SpeI site in the origin of replication is: | ||
| - | CAA CTC AAA GGA AAA GGA CTA ATA ATT ATC ATT GAC TAG | + | 5' CAA CTC AAA GGA AAA GGA CTA ATA ATT ATC ATT GAC TAG 3' |
Latest revision as of 21:33, 24 October 2007
We made the site directed mutagenesis with help of PCR. The sequence for the Primer to "kill" the SpeI site in the origin of replication is:
5' CAA CTC AAA GGA AAA GGA CTA ATA ATT ATC ATT GAC TAG 3'
