USTC

From 2007.igem.org

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In more than 4 months, our
In more than 4 months, our
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<font color="#bb0000">'''Synthesis'''</font>
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'''Parts designed'''<br>
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= <font color="#bb0000">'''229'''</font> parts<br>
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'''Parts submitted'''<br>
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= <font color="#bb0000">'''123'''</font> parts
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'''Oligo-synthesis'''<br>
'''Oligo-synthesis'''<br>
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~ 100 strands<br>
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~ 120 fragments<br>
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'''Fluorescence detection'''<br>
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'''PCR synthesizing and detection'''<br>
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> 100 times<br>
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~ 800 times<br>
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'''DNA sequencing'''<br>
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'''Restriction digestion'''
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~ 130 times
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> 600 times
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<font color="#bb0000">'''Detection'''</font>
 
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'''Colony-PCR detection'''<br>
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'''DNA sequencing'''<br>
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> 500 clones<br>
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~ 130 times
'''Fluorescence detection'''<br>
'''Fluorescence detection'''<br>
> 100 times<br>
> 100 times<br>
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'''DNA sequencing'''<br>
 
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~ 130 times
 
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<font color="#bb0000">'''Measurement'''</font>
 
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'''Fluorescent Density measuring'''<br>
'''Fluorescent Density measuring'''<br>
> 200 times<br>
> 200 times<br>
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~ 70 times<br>
~ 70 times<br>
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<!--- <font color="#bb0000">'''Synthesis'''</font> --->
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Revision as of 17:06, 25 October 2007


USTC Logo.png

Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->

Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small piece of DNA containing cis-acting elements and favored for its small scale and potential to implement complicated logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specified DNA-recognition regions are able to transmit signals without mutual interference, just as copper wires. In this way, a circuit can be constructed regardless of the number of logic gates and the manner of connections between the wires.

A demonstration system has also been assembled to show the practicality of this method.Just like Digital Electronic Circuits in early days, it is simple and ugly. But how will it appear in future?


DSCN1243.png

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian


Project

For detailed description, please click each hyperlink below.


Introduction


Core Logic Components:

Logic-Gate Promoters


Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico


Peripheral Devices:

Inputs and Outputs


Extensible System:

A Demonstration

Further More


Members


4 Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu


3 Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun



3 Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Statistics

In more than 4 months, our

Parts designed
= 229 parts
Parts submitted
= 123 parts


Oligo-synthesis
~ 120 fragments
PCR synthesizing and detection
~ 800 times
Restriction digestion > 600 times


DNA sequencing
~ 130 times Fluorescence detection
> 100 times
Fluorescent Density measuring
> 200 times
ONPG assay
~ 70 times


Resources

Modeling Utilities

Protocols

Sponsors

Service Providers

Gallery

Team Photos

Laboratory

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]

Links

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]

[http://partsregistry.org iGEM Standard Parts' Catalog ]

IGEM2007 Team List

[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]

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