Berkeley LBL/JoyceNotebook
From 2007.igem.org
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'''Contruction of pET3a Derivatives Containing ''bchD'' and ''bchI''''' | '''Contruction of pET3a Derivatives Containing ''bchD'' and ''bchI''''' | ||
| - | 1. Amplify Rhodobacter's gene and introduce restriction sites, which allow cloning PCR fragments into pET3a | + | 1. Amplify Rhodobacter's gene and introduce restriction sites by [[Berkeley_LBL/PCRphusion|PCR (Using Phusion Polymerase)]], which allow cloning PCR fragments into pET3a |
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Revision as of 19:07, 25 October 2007
Contruction of pET3a Derivatives Containing bchD and bchI
1. Amplify Rhodobacter's gene and introduce restriction sites by PCR (Using Phusion Polymerase), which allow cloning PCR fragments into pET3a
| bchD | bchI | |
|---|---|---|
| Restriction sites introduced when amplified by PCR | NdeI, BamHI | NdeI, BglI |
