PennState/Lab/Notebooks

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<a href="https://2007.igem.org/BerkiGEM2007Present4"><img border="0" src="https://static.igem.org/mediawiki/2007/thumb/9/92/PennStateiGEM2007Garrett.jpg/500px-PennStateiGEM2007Garrett.jpg" width="121" height="121"></a></td>
<a href="https://2007.igem.org/BerkiGEM2007Present4"><img border="0" src="https://static.igem.org/mediawiki/2007/thumb/9/92/PennStateiGEM2007Garrett.jpg/500px-PennStateiGEM2007Garrett.jpg" width="121" height="121"></a></td>
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<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present4">Garrett's Lab Notebook</a></b><p><i>Our bacterial
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<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present4">Garrett's Lab Notebook</a></b><p></td>
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chassis has been heavily modified to remove its
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sepsis-inducing toxicity, immunogenic factors, and ability to grow
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within the bloodstream, as well as promote its ability to last
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longer in the bloodstream by masking it from the immune
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system.</i></td>
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<a href="https://2007.igem.org/BerkiGEM2007Present3"><img border="0" src="https://static.igem.org/mediawiki/2007/0/00/PennStateiGEM2007Luc.jpg" width="121" height="121"></a></td>
<a href="https://2007.igem.org/BerkiGEM2007Present3"><img border="0" src="https://static.igem.org/mediawiki/2007/0/00/PennStateiGEM2007Luc.jpg" width="121" height="121"></a></td>
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<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present3">Luc's Lab Notebook</a></b><p><i>The Controller
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<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present3">Luc's Lab Notebook</a></b><p></td>
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is an integrated genetic circuit comprised of two plasmids that allows
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stable maintenance of the system's various operons on a large single-copy
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plasmid in a dormant state.  Upon induction, the copy number of the
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operons and their transcription increase 100-fold resulting in a
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dramatic increase in protein expression.</i></td>
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<a href="https://2007.igem.org/BerkiGEM2007Present5"><img border="0" src="https://static.igem.org/mediawiki/2007/thumb/d/dc/PennStateiGEM2007Noah.jpg/500px-PennStateiGEM2007Noah.jpg" width="121" height="121"></a></td>
<a href="https://2007.igem.org/BerkiGEM2007Present5"><img border="0" src="https://static.igem.org/mediawiki/2007/thumb/d/dc/PennStateiGEM2007Noah.jpg/500px-PennStateiGEM2007Noah.jpg" width="121" height="121"></a></td>
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<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present5">Noah's Lab Notebook</a></b><p><i>To
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<td class="tddesc"><b><a href="https://2007.igem.org/BerkiGEM2007Present5">Noah's Lab Notebook</a></b><p></td>
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prevent chance of infection or unwanted proliferation after
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hemoglobin production, we have engineered a genetic
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self-destruct mechanism whereby when induced, the bacterial
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cell will express a genetic material-degrading toxin which
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kills the cell, but leaves it physically intact.</i></td>
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Revision as of 22:58, 25 October 2007

Our Lab Notebooks
Galen's Lab Notebook
Garrett's Lab Notebook
Luc's Lab Notebook
Noah's Lab Notebook
Freeze Drying

To enable preservation of our bacteria for prolonged periods, we are including the ability to produce the compounds hydroxyectoine and trehalose that will enable our bacteria to survive freeze-drying intact.  This will dramatically increase shelf-life and decrease transport costs.

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