Princeton/lab/experimentation
From 2007.igem.org
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Please consult the relevant vendors for protocol documents. | Please consult the relevant vendors for protocol documents. | ||
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| + | Assembly Line Flow | ||
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| + | Design Primers | ||
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| + | PCR | ||
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| + | Run Gel | ||
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| + | Gel Extraction and determine concentrations | ||
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| + | Digestion with Restriction Enzymes | ||
| + | |||
| + | PCR purification and determine concentrations | ||
| + | |||
| + | Ligation | ||
Revision as of 21:53, 26 October 2007
Experimentation
Protocols
For reliability, a majority of our actual experimentation has followed protocols provided by the supplier of relevant projects, including enzymes ([http://www.neb.com/ NEB]) and kits ([http://www.qiagen.com/ Qiagen]). The [http://www.qiagen.com/ Qiagen] maxiprep kit was replaced with a [http://www.bio-rad.com/ Bio-Rad] kit to obtain higher yields with lower processing times.
Please consult the relevant vendors for protocol documents.
Assembly Line Flow
Design Primers
PCR
Run Gel
Gel Extraction and determine concentrations
Digestion with Restriction Enzymes
PCR purification and determine concentrations
Ligation
