Imperial/Dry Lab/Software

From 2007.igem.org

(Difference between revisions)
(Constitutive synthesis of a Protein)
(Constitutive synthesis of a Protein)
Line 6: Line 6:
== Constitutive synthesis of a Protein ==
== Constitutive synthesis of a Protein ==
-
The experimental setup involves tagging a constitutive promoter with a fluorescent protein; we record the resultant fluorescence and do some analysis. This routine loads the data as an excel sheet, allows for graphic visualization of the results, and if need be, elimination of some samples which may seem suspect.  The synthesis rate and degradation term of the milieu are estimated by the user.   
+
The experimental setup involves tagging a constitutive promoter with a fluorescent protein; the resultant fluorescence is then recorded and it is up to the user now to perform some analysis. This routine loads the experimental data as an excel sheet, allows for graphic visualization of the results, and if need be, elimination of some samples which may seem suspect (outliers).  The synthesis rate and degradation term of the milieu are defined/estimated by the user.   
The routine was developed so the user may supervise all the operations.
The routine was developed so the user may supervise all the operations.

Revision as of 01:53, 26 October 2007

Contents

Useful Tools

During this summer the Imperial iGEM team has developed several routines in MATLAB. We are happy to share them with the rest of Synthetic Biology Community and hope they will prove useful.

Analysis of the Degradation of a Protein

Constitutive synthesis of a Protein

The experimental setup involves tagging a constitutive promoter with a fluorescent protein; the resultant fluorescence is then recorded and it is up to the user now to perform some analysis. This routine loads the experimental data as an excel sheet, allows for graphic visualization of the results, and if need be, elimination of some samples which may seem suspect (outliers). The synthesis rate and degradation term of the milieu are defined/estimated by the user. The routine was developed so the user may supervise all the operations.

Infector Detector

Intro in ID + link

1 sub section per piece of code

Description of the code + images if possible ==

Cell-by-Date

Intro in CBD + link

1 sub section per piece of code

Description of the code + images if possible ==