BerkiGEM2007-AustinConstructionFile17

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
-
9145-I716065 -KmR cassette-rbs library-HbA alpha 2X mutant- (Bgl2/EcoR1, 2536/1027, Larger)<br>
+
9145-I716065 -KmR cassette-rbs library-HbA alpha 2X mutant- (BamH1/EcoR1, 2536/1027, Larger)<br>
-
9145-I716066 -Cmr cassette-rbs library-HbA beta 2Xmutant- (BamH1/EcoR1, 2063/1383, Smaller)<br>
+
9145-I716066 -Cmr cassette-rbs library-HbA beta 2Xmutant- (BglII/EcoR1, 2063/1383, Smaller)<br>
-
Ligated Product is 9415-I716067 Select with Cmr <br>
+
Ligated Product is 9415-I716067 (beta-alpha cassette 2X mutant) Select with Kan <br>

Revision as of 16:08, 20 June 2007

9145-I716065 -KmR cassette-rbs library-HbA alpha 2X mutant- (BamH1/EcoR1, 2536/1027, Larger)
9145-I716066 -Cmr cassette-rbs library-HbA beta 2Xmutant- (BglII/EcoR1, 2063/1383, Smaller)
Ligated Product is 9415-I716067 (beta-alpha cassette 2X mutant) Select with Kan